microRNA-222在肝细胞癌组织中的表达及意义  

Expression of microRNA-222 in hepatocellular carcinoma tissue and its clinical significance

作　　者：张竹青[1] 卢书明[2] 王花丽[3] 徐梦杳 李春艳[2] 辛悦[1] 赵俊军[1] 

机构地区：[1]大连市中心医院病理科,辽宁大连116033 [2]大连医科大学附属第一医院消化内科,辽宁大连116011 [3]大连医科大学附属第一医院病理科,辽宁大连116011

出　　处：《临床肝胆病杂志》2017年第8期1502-1505,共4页Journal of Clinical Hepatology

基　　金：国家自然科学基金项目(81502274);辽宁省自然科学基金资助项目(2015020324);大连市卫生局课题

摘　　要：目的检测microRNA-222(miRNA-222)在肝细胞癌(HCC)组织中的表达,并探讨其临床意义。方法收集2012年1月-2014年12月于大连市中心医院、大连医科大学附属第一医院手术切除的HCC组织103例,采用实时定量PCR方法检测HCC、近癌旁肝硬化组织及远癌旁肝组织中miRNA-222的表达水平,分析miRNA-222表达与HCC临床病理特征之间的关系。计量资料多组间比较采用单因素方差分析,进一步两两比较采用Dunnett's T3法;两组间比较采用t检验。结果 miRNA-222在HCC、近癌旁肝硬化组织及远癌旁肝组织中的表达差异有统计学意义(F=27.167,P<0.001),其在HCC组织中的相对表达量(4.543±2.029)明显高于在近癌旁肝硬化组织(3.072±0.844)及远癌旁肝组织(1.559±0.695)中的相对表达量(P值均<0.01);近癌旁肝硬化组织中miRNA-222的相对表达量显著高于远癌旁肝组织(P<0.01)。miRNA-222的表达与HCC的大小、分化程度、门静脉瘤栓、侵袭转移均有关联(t值分别为7.256、9.900、2.345、4.225,P值均<0.05);而与性别、年龄、合并病毒性肝炎及AFP升高无关(P值均>0.05)。结论 miRNA-222在HCC组织中异常高表达,检测miRNA-222有助于HCC临床进展及预后的判断。Objective To investigate the expression of microRNA-222（ miRNA-222） in hepatocellular carcinoma（ HCC） tissue and its clinical significance. Methods A total of 103 HCC tissue samples were collected from the patients who underwent surgical resection in Dalian Central Hospital and The First Affiliated Hospital of Dalian Medical University from January 2012 to December 2014. Quantitative real-time PCR was used to measure the expression of miRNA-222 in HCC tissues,adjacent cirrhotic tissues,and distal liver tissues,and the association between miRNA-222 expression and the clinicopathological features of HCC was analyzed. A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the Dunnett＇s T3 test was used for further comparison between any two groups; the t-test was used for comparison between two groups. Results There was a significant difference in the expression of miRNA-222 between HCC tissues,adjacent cirrhotic tissues,and distal liver tissues（ F = 27. 167,P 0. 001）; the HCC tissues had significantly higher relative expression of miRNA-222 than the adjacent cirrhotic tissues（ 4. 543 ± 2. 029 vs 3. 072 ± 0. 844,P 0. 01） and the distal liver tissues（ 4. 543 ± 2. 029 vs 1. 559 ± 0. 695,P 0. 01）,and the adjacent cirrhotic tissues had significantly higher relative expression of miRNA-222 than the distal liver tissues（ P 0. 01）. The expression of miRNA-222 was significantly associated with tumor size,degree of tumor differentiation,tumor thrombus in the portal vein,and invasion/metastasis（ t = 7. 256,9. 900,2. 345,and 4. 225,all P 0. 05）,while it was not associated with sex,age,the presence of viral hepatitis,or increased alpha-fetoprotein level（ all P 0. 05）. Conclusion The expression of miRNA-222 is abnormally upregulated in HCC tissue,and miRNA-222 measurement may help to evaluate the clinical progression and prognosis of HCC.

关 键 词：癌 肝细胞 微RNAS 

分 类 号：R735.7[医药卫生—肿瘤]