Surface chemistry of carbon nanoparticles functionally select their uptake in various stages of cancer cells  

作　　者：Indrajit Srivastava Santosh K. Misra Fatemeh Ostadhossein Enrique Daza Jasleena Singh Dipanjan Pan 

机构地区：[1]Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA [2]Biomedical Research Center, Carle Foundation Hospital, Urbana, Illinois 61801, USA [3]Department of Materials Science and Beckman Institute, University of illinois, Urbana-Champaign, Urbana, Illinois 61801, USA

出　　处：《Nano Research》2017年第10期3269-3284,共16页纳米研究（英文版）

摘　　要：Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal application. Here, we report the systematic synthesis of sub-50 nm carbon nanoparticles （CNP） presenting neutral, anionic, and cationic surface functionalities. A subset of CNPs with -10, 20, and 40 nm hydrodynamic sizes were synthesized with neutral surface headgroups. For the first time, the cellular internalization of these CNPs was systematically quantified in various stages of breast cancer cells （early, late, and metastatic）, thereby providing a parametric assessment of charge and size effects. Distinct activities were observed when these systems interacted with cancer cells in various stages. Our results indicated that metastatic breast cancer could be targeted by a nanosystem presenting anionic phosphate groups. On the contrary, for patients in late stage of cancer, drugs could be delivered with sulfonate functionalized carbon nano- particles, which have higher probability of intracellular transport. This study will facilitate the better understanding of nanoparticle-biological entity interaction, and the integration of this knowledge with pathophysiology would promote the engineering of nanomedicine with superior likelihoods of crossing the endocytic ＂barrier＂ for drug delivery inside cancerous cells.Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal application. Here, we report the systematic synthesis of sub-50 nm carbon nanoparticles （CNP） presenting neutral, anionic, and cationic surface functionalities. A subset of CNPs with -10, 20, and 40 nm hydrodynamic sizes were synthesized with neutral surface headgroups. For the first time, the cellular internalization of these CNPs was systematically quantified in various stages of breast cancer cells （early, late, and metastatic）, thereby providing a parametric assessment of charge and size effects. Distinct activities were observed when these systems interacted with cancer cells in various stages. Our results indicated that metastatic breast cancer could be targeted by a nanosystem presenting anionic phosphate groups. On the contrary, for patients in late stage of cancer, drugs could be delivered with sulfonate functionalized carbon nano- particles, which have higher probability of intracellular transport. This study will facilitate the better understanding of nanoparticle-biological entity interaction, and the integration of this knowledge with pathophysiology would promote the engineering of nanomedicine with superior likelihoods of crossing the endocytic ＂barrier＂ for drug delivery inside cancerous cells.

关 键 词：personalized medicine ENDOCYTOSIS surface charge size carbon nanoparticles 

分 类 号：TQ174.758[化学工程—陶瓷工业] Q279[化学工程—硅酸盐工业]