丙戊酸钠诱导多发性骨髓瘤细胞株细胞凋亡和自噬  

Effects of apoptosis and autophagy in multiple myeloma cell lines induced by valproic acid

作　　者：裴晓川 张媛媛[1] 赵瑞娟[1] 白春强 王丽红[1] 李俊东[1] 李红[1] 闫丽娜[1] 张志华[1] 郝长来[1] PEI Xiaochuan ZHANG Yuanyuan ZHAO Ruijuan et al(Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, CHIN)

机构地区：[1]承德医学院附属医院血液内科,河北省067000

出　　处：《江苏医药》2017年第16期1133-1136,F0002,共5页Jiangsu Medical Journal

基　　金：河北省自然科学基金(H2013406112)

摘　　要：目的探讨丙戊酸钠(VPA)对多发性骨髓瘤(MM)细胞株细胞凋亡与自噬的影响。方法 MM细胞株RPMI8226和U266分为空白对照组及VPA 2、4、8mmol/L三个处理组。HE染色法观察VPA诱导细胞凋亡的形态学改变;流式细胞术检测VPA诱导RPMI8226和U266细胞凋亡情况。实时定量PCR和Western blot检测凋亡信号传导通路蛋白Bcl-2、Bax、Caspase-3和Caspase-8 mRNA和蛋白表达。结果随着VPA浓度升高,RPMI8226和U266细胞凋亡率上升(P<0.05)。VPA浓度为8mmol/L时,Bax、Caspase-3、Caspase-8 mRNA和蛋白相对表达量随着VPA作用时间延长而增加,而Bcl-2 mRNA和蛋白相对表达量随VPA作用时间延长而下降(P<0.05)。结论 VPA促进MM细胞株RPMI8226和U266细胞凋亡,且呈浓度和时间依赖性。Objective To explore the effects of apoptosis and autophagy in multiple myeloma（MM）cell lines induced by valproic acid（VPA）.Methods RPMI8226 and U266 cells of MM cell lines were divided into groups of blank control,and VPA of 2,4 and 8 mmol/L.Morphological changes of cell apoptosis induced by VPA were observed through HE staining,cell apoptosis of which was evaluated by flow cytometry,and mRNA and protein expressions of Bcl-2,Bax,Caspase-3,Caspase-8 in apoptosis signaling pathways were detected by real-time quantitative PCR and Western blot.Results Along with VPA concentration increasing,cell apoptosis rates of RPMI8226 and U266 were increased（P〈0.05）.When VPA was 8 mmol/L,mRNA and protein expressions of Bax,Caspase-3,and Caspase-8 were increased along with prolonging cultured time of VPA,while Bcl-2 mRNA and protein expressions were decreased（P〈0.05）.Conclusion VPA can promote cell apoptosis in MM cell lines RPMI8226 and U266 in the dose-and time-dependent manners.

关 键 词：多发性骨髓瘤 丙戊酸钠 细胞凋亡 自噬 

分 类 号：R733[医药卫生—肿瘤]