Genome-wide identification of long noncoding RNAs in myocyte differentiation  

基于高通量测序的骨骼肌分化相关长非编码RNA的鉴定(英文)

作　　者：杨琴[1] 李栋[1] 冯超[1] 易凡[1] 李华[2] 杜权[1] 

机构地区：[1]北京大学医学部药学院天然药物及仿生药物国家重点实验室,北京100191 [2]北京大学第三医院妇产科,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2017年第6期423-431,共9页中国药学（英文版）

基　　金：Beijing Natural Science Foundation(Grant No.217 1001);the National Natural Science Foundation of China(Grant No.31571403);the National High-tech R&D Program of China(G rant No.2014AA021103)

摘　　要：Taking advantage of high-throughput sequencing technology, a large number of long noncoding RNAs （lncRNAs） have been recently characterized. Emerging evidence suggests that they play critical roles in diverse biological processes, including the differentiation of skeletal muscle cells. As previous studies have focused mainly on the polyadenylated lncRNAs, the involvement of polyA-rninus IncRNAs in myocyte differentiation remains largely unexplored. To this aspect, their expression and regulation were examined in the present study. During the course of myocyte differentiation of C2C12 cells, polyA-minus RNAs were isolated and analyzed by RNA-seq. In addition to identifying 904 novel polyA-minus lncRNAs, their temporal expression was further characterized in this process. For many lncRNAs, differentiation-specific profiles were revealed. Based on their unique expression profiles, these lncRNAs were grouped into nine regulation categories. Taken together, our study greatly contributed to the identification of a dynamic regulation ofpolyA-minus lncRNAs, and clarified their potential roles in myocyte differentiation.在细胞的生长、分化及个体发育过程中,长非编码RNA均发挥了重要的调控作用。然而在以往的研究中,人们主要关注蛋白编码基因的功能,对长非编码RNA的了解非常有限。为了研究polyA-minus长非编码RNA对骨骼肌细胞分化的意义,本研究以小鼠C2C12成肌细胞为研究对象,利用polyA-minus RNA反向富集技术和高通量RNA测序,分析了这类基因转录本在该过程中的表达变化,旨在发现具有调控作用的长非编码RNA基因。在本工作中,我们不仅鉴定了904条新的长非编码RNA转录本,还阐明了这些长非编码RNA和蛋白编码基因在各关键分化节点的表达调控情况,并进一步将这些长非编码RNA分为9种不同的调控类型。我们获得的研究成果有可能为肌肉疾病的治疗提供新的分子靶标。

关 键 词：LncRNA RNA-SEQ Myocyte differentiation 

分 类 号：R68[医药卫生—骨科学]