大鼠骨折模型局部注射C型钠尿肽(CNP)对骨折愈合的影响  

The Influence of Local Injection of C-type Natriuretic Peptide on Fracture Healing in a Rat Model

作　　者：宋正鑫[1] 祝腾蛟 刘冰川[1] 周方[1] 田耘[1] 

机构地区：[1]北京大学第三医院骨科,北京100083

出　　处：《中国微创外科杂志》2017年第9期827-831,共5页Chinese Journal of Minimally Invasive Surgery

摘　　要：目的探讨局部注射C型钠尿肽(C-type natriuretic peptide,CNP)对于大鼠骨折的愈合是否具有促进作用。方法 36只12周龄雄性SD大鼠,建立大鼠股骨干骨折模型,随机分为3组,每组12只。组1大鼠在断骨后,在骨折处局部注射溶于1 ml凝胶中的25μg CNP;组2大鼠在断骨后,在骨折处局部注射凝胶1 ml;组3大鼠在断骨后,在骨折处局部注射医用生理盐水1 ml。在建模后每周对大鼠进行X线扫描检测。第3周时,随机从每组中各选取7只大鼠,在接受X线扫描后处死,股骨接受Micro-CT扫描检测。每组剩余的5只大鼠在第4周接受X线扫描后处死,股骨接受Micro-CT扫描检测。收集建模后第3、4周股骨及骨折部位样本进行生物力学检测。结果 X线显示组1在术后第3、4周均出现大鼠骨折部位骨桥的形成,组2、3所有大鼠直至实验结束也未出现骨折端有骨桥形成。Micro-CT结果显示术后第3周组1骨折端骨痂体积为(187.655±62.864)mm3,高于组2(137.703±52.592)mm3和组3(176.493±75.193)mm3,但无统计学差异(F=1.167,P=0.334);术后第4周组1、2、3的骨折端骨痂体积分别为(242.853±139.252)、(242.460±64.844)、(226.274±77.418)mm3,3组比较无统计学差异(F=0.045,P=0.956)。组1第3周时4只大鼠达到骨折愈合标准,组2、3未出现骨折愈合大鼠;组1第4周时4只大鼠达到骨折愈合标准,组2、3未出现骨折愈合大鼠。生物力学检测结果显示第3周时,组1中能够完成生物力学检测的大鼠为4只,组2为0只,组3为1只;第4周时,组1中能够完成生物力学检测的大鼠为4只,组2为0只,组3为0只。结论在骨折局部注射CNP对SD大鼠骨折的愈合有一定的促进作用。Objective To evaluate the promoting effect of C-type na triure tic pept ide （ C NP ） on facture heal ing in a rat model. Methods A total of 36 Sprague-Dawley （ SD ） male rats were employed for induction of femoral fracture model in three randomly divided groups （12 for each group）. Rats in Group 1 received local injection of 1 ml gel containing 25 （jug of CNP, rats in Group 2 were treated with local injection of 1 ml of gel, and rats in Group 3 were provided with 1 ml of saline solution at the same position. The X-ray was performed for each group after fracture at each week （0 , 1 s t , 2 n d , 3 rd , and 4 th we eks） . F u r th e rm o re , legs of 7 rats in each group were harvested in the 3rd week and the remaining 5 rats in each group were obtained in the 4th week. The progression of fracture healing of each specimen was assessed by micro-CT. Mechanical tests of the femora and fractures were carried out at the 3rd and 4th weeks. Results The X-ray showed that some rats of the Group 1 ap pe ared the formation of bone bridge at the site of fracture at 3rd and 4th weeks after surgery, while the Group 2 and 3 did not appear bone bridge at the end of experiment in all rats. The results of micro-CT showed that the volume of callus in the Group 1 at the fracture site at 3rd week was （ 187.655 ± 62. 864 ） mm3 , which was higher than that in the Group 2 （ 137. 703 ± 52. 592） mm3 and Group 3 （ 176. 493 ± 75. 193） mm3 , but the differences were not statistically significant （F = 1.167, P = 0.334）. At the 4th week, the volumes of callus in the Group 1,2, and 3 were （242.853 ± 139.252） mm3, （242.460 ±64. 844） mm3, and （226.274 ±77.418） mm3, respectively, and there were no significant differences among the 3 groups （F = 0.045, P= 0.956）. Four rats in the Group 1 reached the standard of fracture healing at the 3rd week, but none in the Group 2 or Group 3. At the 4th week, there were another 4 rats reaching the standard of fracture healin

关 键 词：骨折愈合 C型钠尿钛 动物模型 

分 类 号：R-332[医药卫生] R683