菊粉联合游泳训练对代谢综合征大鼠骨骼肌胰岛素受体底物1及葡萄糖转运子4表达的影响  

Effect of inulin combined with swimming training on expression of IRS-1,GluT4 in skeletal muscle of metabolism syndrome rats

作　　者：赵金香 胡章云[2] 李耀华[3] 李燕玲[3] 谢萍[3] 高奋堂[3] 

机构地区：[1]甘肃医学院,平凉744000 [2]和政县人民医院心内科 [3]甘肃省人民医院心内科

出　　处：《中国糖尿病杂志》2017年第9期831-835,共5页Chinese Journal of Diabetes

基　　金：甘肃省生卫生行业科研计划项目(GSWSKY-2015-04)

摘　　要：目的观察菊粉联合游泳训练对代谢综合征(MS)大鼠骨骼肌胰岛素受体底物1(IRS-1)、葡萄糖转运体子4(GluT4)表达的影响。方法高糖高脂高盐喂养结合腹腔注射STZ建立MS大鼠模型,干预8周后检测各组FBG、HbA_1c及FIns,计算HOMA-IR;HE染色法观察各组肝脏病理变化;免疫印迹及免疫组织化学法检测大鼠骨骼肌IRS-1、GluT4表达。结果与正常饮食对照组比较,模型组FBG、HbA_1c、FIns及HOMA-IR升高(P<0.01),肝脏出现非酒精性脂肪性肝病(NAFLD)病理改变,骨骼肌IRS-1、GluT4表达降低(P<0.01)。与模型组比较,菊粉联合游泳训练组FBG、HbA_1c、FIns及HOMA-IR降低(P<0.01),NAFLD减轻,骨骼肌IRS-1、GluT4表达升高(P<0.01)。结论菊粉联合游泳训练降低了MS大鼠FBG、HbA_1c及FIns水平,减轻了NAFLD病理改变,改善IR,其机制可能与上调骨骼肌IRS-1、GluT4表达有关。Objective To investigate the effect of inulin combined with swimming training on the expression of IRS-1 and GluT4 in skeletal muscle of metabolic syndrome rats. Methods The rats model of metabolic syndrome （MS） were established by feeding with high fat, high sugar and high salt diet and injected streptozocin（STZ） intraperitoneally. FBG, HbA1c and Fins were detected. HOMA-IR was calculated after eight weeks of intervention. HE staining method was used to observe the pathological changes of liver. Western blot and immune histochemical methods were used to detect the expression of IRS-1 and GluT4 in skeletal muscle. Results Compared with the control group, the liver of model group presented the pathological changes of Nonalcoholic Fatty Liver Disease （NAFLD）, the levels of FBG, HbA1 e,Flns and HOMA-IR were significantly increased （P〈0.01）, the expressions of IRS-1 and GluT4 in skeletal muscle were significantly decreased （P〈 0.01）. Compared with the model group, the pathological changes of NAFLD in inulin combined with swimming training group significantly were alleviated,, the levels of FBG, HbA1c, Fins and HOMA-IR were significantly decreased （P〈 0.01）, the expression of IRS-1 and GluT4 in skeletal muscle were significantly increased （P〈0.01）. Conclusion Inulin combined with swimming training can alleviate the pathological changes of NAFLD and improve insulin resistance in rats with MS by upregulating the expression of IRS-1 and GluT4 in skeletal muscle.

关 键 词：菊粉 游泳训练 胰岛素抵抗 胰岛素受体底物1 葡萄糖转运体4 

分 类 号：R589[医药卫生—内分泌]