经股动脉注射途径建立前列腺癌和乳腺癌骨转移模型  

Establishment of mouse model of bone metastasis of prostate cancer and breast cancer via femoral artery injection of tumor cells

作　　者：殷昭阳 齐思勇 成祥[3] 郭靓[3] 陈泓宇[3] 施明[3] 

机构地区：[1]武警山东省总队莱芜市支队卫生队,山东莱芜271199 [2]漯河市中心医院泌尿外科,河南漯河462000 [3]解放军军事医学科学院基础医学研究所,北京100850

出　　处：《南方医科大学学报》2017年第7期914-918,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81572845;81402562)~~

摘　　要：目的探索建立高效、可靠的肿瘤骨转移动物模型的方法。方法将C57BL/6J雄鼠随机分为4组,每组15只;BALB/c雌鼠15只,设一组。将小鼠前列腺癌细胞RM-1和乳腺癌细胞4T1-luc消化、洗涤、计数后用生理盐水重悬至1×105/m L,取100μL,经股动脉将4T1 luc细胞注射到BALB/c雌鼠,并通过活体成像检验此方法转移成瘤的特异性。将100μL相同浓度的RM-1细胞悬液分别经股动脉、髂外动脉注射C57雄鼠各15只,比较两种不同注射方式所需时间、小鼠存活率,注射21天后将下肢骨HE切片,显微镜下观察并比较两种方法建立骨转移模型的成瘤率。结果经股动脉途径注射肿瘤细胞悬液所需的止血时间为2.53±1.75 min,经髂外动脉注射方法所需的止血时间为4.70±1.63 min(P<0.05);经股动脉注射方法的手术时间为14.67±2.16 min,经髂外动脉注射方法的手术时间为22.47±3.50 min(P<0.05);术后21 d经股动脉和髂外动脉注射组小鼠的生存率分别为93.3%和66.7%(P<0.05);采用两种方法的肿瘤转移率均为100%;分别经股动脉和髂外动脉注射生理盐水组和肿瘤细胞悬浮液组在上述3项指标无显著差异。活体成像结果显示,肿瘤特异性地转移到了股骨和胫骨中。结论经股动脉注射方法建立肿瘤骨转移模型的手术及止血时间短、小鼠的存活率及肿瘤转移的成功率、特异性高,可作为肿瘤骨转移的新型动物模型。Objective To explore an efficient and reliable method for establishing an animal model of bone metastasis of tumors. Methods Male C57BL/6J mice were randomized into 4 equal groups to receive injections of normal saline or prostate cancer RM-1 cell suspension via the femoral artery or the external iliac artery, and breast cancer 4T1-luc cells were injected in 15 female BALB/c mice via the femoral artery. The operation time, postoperative survival rate, and the tumor formation rates in the mice with two different injection methods were compared. The tumor metastasis in the mice was evaluated with in vivo imaging. Results The mean time for hemostasis time was 2.53 ＆#177; 1.75 min in mice receiving tumor cell injection via the femoral artery, significantly shorter than that in mice with injections via the external iliac artery （4.70 ＆#177; 1.63 min; P〈0.05）; the mean operation time was 14.67＆#177;2.16 min and 22.47＆#177;3.50 min in the two groups, respectively （P〈0.05）. At 21 days after the operation, the survival rate was 93.3% in femoral artery injection group, significantly higher than that in external iliac artery injection group （66.7%; P〈0.05）. The tumor metastasis rate was 100% in both groups. Conclusion Injection of the tumor cells via the femoral artery is more suitable for establishing mouse models of bone metastasis of cancers.

关 键 词：前列腺癌 乳腺癌 骨转移 动物模型 

分 类 号：R-332[医药卫生] R737.25R737.9