探讨两种实验方法在检测产碳青霉烯酶肠杆菌科细菌中的临床应用  

Study on the Clinical Application of Two Experimental Methods for Carbapenemase-producing Enterobacteriaceae

作　　者：杨钰欣[1] 闵宗素 付甫海 刘天艳[1] 

机构地区：[1]遵义市妇幼保健院检验科,贵州遵义563000

出　　处：《系统医学》2017年第13期9-12,共4页Systems Medicine

基　　金：遵义市福建发改关项目(遵义市科合社字[2014]48号)

摘　　要：目的评价改良Hodge试验(MHT)及Carba NP试验(CNPt)在检测产碳青霉烯酶肠杆菌科细菌中的临床应用价值,以确立最佳的筛选方法。方法收集该院2014年3月—2016年3月经法国生物梅里埃vitek2 compact全自动药敏鉴定系统所鉴定的对碳青霉烯类抗生素耐药的肠杆菌科细菌80株。用荧光定量PCR检测其碳青霉烯酶基因blaKPC、blaNDM、blaOXA-48、blaIMP、blaVIM、blaSME;再用MHT及CNPt对所收集的细菌进行碳青霉烯酶检测。结果 MHT检出阳性菌株51株,CNPt检出阳性菌株72株,分别与PCR结果进行比较,CNPt与耐药基因检测结果符合率更高。结论 CNPt可代替MHT作为检测碳青霉烯酶的筛选方法,应用于临床。Objective This paper tries to evaluate the clinical application of the modified hodge test （MHT） and Carba NP test （CNPt） on carbapenemase-producing enterobacteriaceae and to decide the best screening method. Methods 80 cases of enterobacteriaceae resistant to carbapenem antibiotics in this hospital from March 2014 to March 2016 were i-dentified by French bioMerieux vitek2 compact automatic drug susceptibility identification system. Carbapenemase genes including blaKPC, blaNDM, blaOXA-48, blaIMP, blaVIM, and blaSME were tested by fluorescent quantitative PCR, and then detected the carbapenemase-producting strains by MHT and CNPt. Results There were 51 positive strains detected by MHT, 72 positive strains detected by CNPt, both results were compared with that of PCR, and the coincidence rate of CNPt was higher than that of MHT. Conclusion The CNPt can be used as screening method for carbapenemase-producing enterobacteriaceae and replace the MHT method, so it is worth clinical application.

关 键 词：改良Hodges试验 CarbaNP试验 碳青霉烯酶基因 

分 类 号：R-331[医药卫生]