黄金茶多糖超声提取工艺及体外抗氧化研究  

A Study on Ultrasonic Extraction Technology for Gold Tea Polysaccharide and Its Ecto Antioxidant Activity

作　　者：熊磊[1] 陈慧[1] 胡文兵[1] 杨占威 王文君[1] 

机构地区：[1]江西农业大学食品科学与工程学院/南昌市农产品加工与质量控制重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2017年第4期801-809,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31560459);江西省自然科学基金项目(20151BAB204037);江西省高等学校科技落地计划资助项目(KJLD13027);江西省教育厅科技计划项目(GJJ13281);广东省农产品加工重点实验室开放基金(201603);江西省研究生创新专项资金项目(YC2016-S186)~~

摘　　要：研究超声波提取黄金茶多糖工艺及体外抗氧化活性。采用L9(34)正交试验、方差分析和多重比较法对超声波提取黄金茶多糖进行试验和结果分析;对黄金茶多糖提取的单因素(超声功率、超声时间、料液比、超声温度)进行优化,通过测定黄金茶多糖清除自由基能力和还原能力来评价其抗氧化活性。黄金茶多糖优化提取工艺为超声功率165 W,超声时间40 min,料液比1∶40,超声温度65℃;黄金茶多糖最高得率可达11.23%。黄金茶多糖对DPPH、·OH、超氧阴离子自由基清除能力和还原能力低于Vc,差异极显著(P<0.01);0.2 mg/m L多糖和VC对ABTS+自由基清除能力,二者差异极显著(P<0.01);黄金茶多糖对·OH、ABTS+自由基的半抑制浓度(IC50)分别为1.713 mg/m L、0.553 mg/m L。黄金茶中多糖的含量较高,在一定浓度范围内,多糖浓度越高,其抗氧化活性越强。To study the extraction technology for gold tea polysaccharide and its antioxidant activity in vitro.L9(3~4) orthogonal test was performed to extract gold tea polysaccharides by ultrasonic extraction method,and the results were analyzed by variance analysis and multiple comparison method.The single factors(ultrasonic power,ultrasonic time,solid-liquid ratio,ultrasonic temperature) were optimized,and the antioxidant activity was evaluated by measuring the free radical scavenging ability and the reducing capacity of gold tea polysaccharide.The optimum extraction conditions were ultrasonic power 165 W,ultrasonic time 40 min,ratio of material to liquid 1∶40,ultrasonic temperature 65 ℃. The highest yield of gold tea polysaccharide was 11.23%. The scavenging ability of DPPH,·OH and superoxide anion radical and the reducing capacity of gold tea polysaccharide were lower than those of Vc(P < 0.01). In comparison with Vc,0.2 mg/m L of gold tea polysaccharide had a significant difference in free radical scavenging ability of ABTS+.The half-inhibiting concentrations of gold tea polysaccharide to IC50 of ·OH and ABTS+radicals were 1.713 and 0.553 mg/m L,respectively(P<0.01).The content of polysaccharide in gold tea is high,the higher the polysaccharide concentration is,the stronger the antioxidant activity is.

关 键 词：黄金茶 多糖 超声波 正交设计 抗氧化性 

分 类 号：TS271[轻工技术与工程—农产品加工及贮藏工程] TQ28[轻工技术与工程—食品科学与工程]