乳腺癌中不同克隆号HER-2抗体IHC与FISH检测结果比较  

Compare the results of HER-2 immunostaining among different clone numbers and with the results of FISH detection in breast cancer

作　　者：房爱菊[1] 张晓莹[1] 程凤凤[1] 戴宗燕 于静 

机构地区：[1]山东省交通医院病理科,济南250031 [2]济南齐鲁医学检验所,济南250031

出　　处：《国际检验医学杂志》2017年第17期2357-2359,共3页International Journal of Laboratory Medicine

基　　金：山东省医药卫生科技发展计划(2015WS0264)

摘　　要：目的 3种不同克隆号人表皮生长因子受体2(HER-2)抗体的免疫组织化学(IHC)检测结果与荧光原位杂交(FISH)检测结果进行比较,为实验室选择合适的HER-2抗体提供依据。方法选择3种不同克隆号的HER-2抗体,对268例浸润性乳腺癌标本进行检测,并与HER-2基因的荧光原位杂交检测进行比较。结果 HER-2的3种抗体的结果分别为克隆号SP3IHC结果3+66例;克隆号EP3IHC结果3+53例;Polyclonal Her-2抗体IHC结果3+80例,差异有统计学意义(P<0.05)。与FISH结果的一致性Kappa值分别为0.76、0.67、0.56。结论 HER-2的IHC检测以选择单克隆抗体优先,建议优先选择克隆号SP3抗体。Objective To compare the immunostaining of human epidermal growth factor receptor-2(HER-2)among three different clones,and to compare with the results of fluorescence in situ hybridization(FISH)detection,to provide evidences for pathological department to select appropriate antibodies.Methods 268 cases of invasive breast cancer were examined by immunohistochemistry using antibodies for HER-2of 3different clones.The results were compared with those done by FISH.Results Immunostaining using antibody clone SP3 showed 3+ reactions in 66cases;Immunostaining using antibody clone EP3 showed 3+ reactions in 53 cases,while using polyclonal HER-2antibody,80 cases showed 3+reactions.There was statistical significance between those groups using different clones(P<0.05).Consistent with the FISH results,the Kappavalues are 0.76,0.67,and 0.56,respectively.Conclusion It suggests detection of HER-2using immunohistochemistry should prioritize selecting monoclonal antibodies,especially clone SP3.

关 键 词：乳腺癌 HER-2抗体 克隆号 荧光原位杂交 

分 类 号：R730.43[医药卫生—肿瘤] R737.9[医药卫生—临床医学]