拟南芥AGO基因家族分析及盐胁迫下的表达验证  

Bioinformatical and experimental analysis of AGO genes in response to salt stress

作　　者：岳路明 宋剑波[1,2,3] 徐晓峰[4] 杨海奇[1] 莫小为[4] 宋军[2] 莫蓓莘[1] Yue Luming Song Jianbo Xu Xiaofeng Yang Haiqi Mo Xiaowei Song Jun Mo Beixin(） Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P. R. China ） College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, Guangdong Province, P. R. China ） College of Science, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, P. R. China ） Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P. R. China)

机构地区：[1]深圳大学生命与海洋科学学院深圳市微生物基因工程重点实验室,广东深圳518060 [2]深圳大学光电工程学院,广东深圳518060 [3]江西农业大学理学院,江西南昌330045 [4]深圳大学生命与海洋科学学院广东省表观遗传学重点实验室,广东深圳518060

出　　处：《深圳大学学报（理工版）》2017年第4期352-357,共6页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(31571332;31560076)~~

摘　　要：argonaute(AGO)蛋白家族成员在小核糖核酸(RNA)介导的转录后调控中有重要作用.通过拟南芥AGO蛋白家族的结构域及各类植物中的AGO蛋白的进化关系进行分析,发现AGO蛋白在植物中结构和进化相对保守.根据已有的芯片数据,对拟南芥中AGO蛋白家族的组织表达进行分析,发现某些AGO基因具有组织表达的特异性.进一步通过基因上游启动子响应元件分析,以及盐等胁迫下的芯片和反转录聚合酶链式反应(RT-PCR)实验数据分析,发现AGO2、AGO3和AGO7等基因受到盐等非生物胁迫的诱导表达.初步探明了拟南芥中AGO基因家族在盐胁迫下的差异表达规律.AGO family proteins play an important role in small RNA-mediated post-transcriptional regulation.Analyzing the domain and evolution of AGO family proteins in diverse plant species,we revealed that the structural organization of AGOs is evolutionally conserved between different plant species. Based on the experimental data,some AGO genes were specifically expressed in various tissues in Arabidopsis. Sequence analysis showed that there are abiotic stress related cis-acting elements in the promotors of AGO genes,microarray and RT-PCR data also indicated the expressions of AGO2,AGO3 and AGO7 genes are induced under salt and other abiotic stress conditions.This is the first study to demonstrate the differential expression patterns of AGOs under salt stress in Arabidopsis.

关 键 词：植物生理学 拟南芥 小核糖核酸 argonaute蛋白 转录后调控 非生物胁迫 

分 类 号：Q946.1[生物学—植物学]