兔脑缺血后自噬激活与ROCK-JNK信号通路的关系  

The relationship between cerebral ischemia autophagy activation with ROCK-JNK signaling pathway

作　　者：王子珍[1] 郑传宜[1] 杨堃[1] 

机构地区：[1]海南医学院第一附属医院神经外科,海南海口570100

出　　处：《中国老年学杂志》2017年第17期4173-4175,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81260371);海南省自然科学基金项目(20158300)

摘　　要：目的探讨脑缺血后神经元自噬激活与Rho蛋白激酶(ROCK)-C-Jun氨基酸末端激酶(JNK)信号通路的关系。方法建立蛛网膜下腔出血(SAH)后脑缺血兔脑模型,检测脑组织梗死面积确定模型是否成功。在二次注血后的不同时间处死模型,取脑缺血区域脑组织,行Western印迹检测脑组织中神经元细胞坏死、凋亡和自噬现象。通过Western印迹检测自噬抗体蛋白在兔脑模型中的表达情况。免疫组化检测脑缺血区域的自噬蛋白和ROCK-JNK信号通路蛋白的表达情况。结果采用Longa线栓法建立SAH模型成功率较高。兔脑组织中存在神经元细胞坏死、凋亡和自噬现象,模型组脑组织梗死面积相比对照组增多。Western印迹及免疫组化证实迟发型血管痉挛与神经元细胞自噬存在关联。结论脑缺血后的脑组织中存在神经元自噬现象且和ROCK-JNK信号通路存在一定关系。Objective To explore the relationship between cerebral ischemia autophagy activation with ROCK-JNK signaling pathway.Methods The subarachnoid hemorrhage cerebral ischemic rabbit brain models were induced to test the extent of brain tissue. After double injection at different times,the rabbits were sacrificed,and brain ischemic area of brain tissue were detected by Western blot.The expressions of ROCK-JNK signaling pathway proteins were detected by immunohistochemical method.Results Neuronal cell necrosis,apoptosis and autophagy phenomenon were observed in rabbit brain tissues. Western blot and immunohistochemistry confirmed the association between late onset vasospasm and neuronal autophagy. Conclusions The neurons autophagy exists in the brain tissue after ischemia,and ROCK-JNK signaling pathway is a certain relationship with autophagy.

关 键 词：脑缺血 ROCK JNK 自噬 

分 类 号：R743.31[医药卫生—神经病学与精神病学]