狗枣猕猴桃叶粗多糖闪式提取工艺及其体外抗氧化活性  

Optimization of Homogenate Extraction of Crude Polysaccharides and Antioxidant Activity From Actinidia kolomikta Leaves

作　　者：常清泉[1] 陆娟[1] 谭莉[1] 王海莲[1] 白晓仙 

机构地区：[1]长春师范大学化学学院,吉林长春130032

出　　处：《北方园艺》2017年第17期149-158,共10页Northern Horticulture

基　　金：吉林省教育厅"十二五"科学技术资助项目(吉教科合字[2013]第255号);长春师范大学自然科学基金资助项目(长师院自科合字政策[2010]第026号);长春师范大学2017年度研究生教育创新计划资助项目(cscxy2017031)

摘　　要：以狗枣猕猴桃叶为试材,以狗枣猕猴桃叶粗多糖提取率为响应值,在单因素试验的基础上,利用响应面法研究闪式提取因素对狗枣猕猴桃叶粗多糖提取率的影响,优化狗枣猕猴桃叶粗多糖闪式提取工艺。同时利用体外自由基清除试验测定粗多糖抗氧化活性。结果表明:当液料比为17.38mL·g^(-1),分散机转速为2.22档,温度为44.54℃,提取时间为10.49min时,狗枣猕猴桃叶粗多糖的提取率最高,可达10.69%。在此条件下所得的狗枣猕猴桃叶粗多糖具有较强的总抗氧化能力及自由基清除能力,其能力大小与粗多糖质量浓度呈现一定的正相关关系。Using the leaves of Actinidia kolomikta as test material, taking the yield of crude polysaccharide as the response value, on the basis of single-test, the effect of homogenate extraction factors on crude polysaccharide were designed by the response surface method（RSM） to determined the optimum technology. And the antioxidant capacity of the crude polysaccharide was assessed through radical scavenging test. The results showed that the liquid-solid ratio was 17.38 mL · g-1, the rotate speed was 2.22 gear, the extraction temperature 44. 54 ℃, and the extraction time was 10.49 minutes. Under the condition the yield of the crude polysaccharide from the leaves of Actinidia kolomikta was 10.69%. antioxidant capacity, and polysaccharide. The radical scavenging the antioxidant capacity test showed that the crude polysaccharide had was proportional to the concentration of crude

关 键 词：狗枣猕猴桃 多糖 闪式提取 响应面分析 抗氧化活性 

分 类 号：S665.309.9[农业科学—果树学]