超声微创外科技术加速鼠正畸牙移动过程中microRNA以及组织蛋白酶K的表达  

Expression of MicroRNA and Cathepsin K in the Process of Accelerating Orthodontic Tooth Movement Through Ultrasonic Minimally Invasive Surgical Techniques in Rats

作　　者：李岩[1] 姚燕[1] 

机构地区：[1]山东省聊城市人民医院,山东聊城252000

出　　处：《中国医学创新》2017年第26期16-20,共5页Medical Innovation of China

基　　金：山东省自然科学基金资助项目(ZR2014HL052)

摘　　要：目的:探讨在超声微创外科技术加速大鼠牙移动过程中组织蛋白酶K以及相关microRNA的表达改变。方法:将2月龄Wistar雄性大鼠60只按照随机分为空白对照组(对照组)、常规正畸治疗组(正畸组)和超声微创外科手术联合正畸治疗组(微创组)三组,每组20只。在正畸组中,大鼠上颌左侧第一磨牙与上切牙之间安装正畸装置,将磨牙以力值30 g的力量拉向近中移动;而微创组则为在正畸治疗的同时,在第一磨牙的近远中用超声骨刀切开骨皮质至骨髓质。每组于建立模型加力7 d时将大鼠处死,通过HE染色观察牙周组织的形态学变化并计数压力侧破骨细胞数目;通过Western blot检测牙周组织中组织蛋白酶K的表达改变;并通过实时定量PCR(Quantitative real time PCR,q RT-PCR)检测牙周组织中维持高表达的microRNA(miR-17,miR-21,miR-126,miR-32,miR-147,miR-155)的表达改变。结果:HE染色结果显示,与对照组相比,正畸组中牙周组织的形态发生明显病理学变化,并且大鼠压力侧破骨细胞数量明显高于对照组(P<0.05)。此外,正畸组中组织蛋白酶K的表达也出现明显上调,并伴随有牙周组织中miR-17、miR-21、miR-126、miR-32、miR-147、miR-155的表达紊乱(P<0.05)。但是在微创组中,牙周组织的形态、大鼠压力侧破骨细胞数量、组织蛋白酶K的表达及microRNA的表达紊乱均得到了改善(P<0.05)。结论:超声微创外科技术创伤小,术后肿痛轻,对牙周组织的影响小,能够加速正畸牙移动,适用于临床正畸治疗。Objective： To explore the expression of cathepsin K and relevant microRNA in the process of accelerating orthodontic tooth movement through uhrasonie minimally invasive surgical techniques in rats. Method ： A total of 60 Wistar male 2-month-old rats were divided into normal control group （ the control group ）, orthodontic device group （ the orthodontic group ） and ultrasonic minimally invasive surgical techniques combined orthodontic treatment group （ the minlmally invasive group ） according to random number table method, 20 cases in each grouo.In the orthodontic group, the rats were installed orthodontic device between the left maxillaryfirst molars and incisors, molars were pulled to the middle with the power of the force value 30 g.The minimally invasive group and the orthodontic group at the same operation, the cortical bone was penetrated to a depth of 0.5 mm mesially and distally in the first molar.The rats of each group were killedon the seventh day.The morphological changes of periodontal tissuewere observedvia HE staining and osteoclast number of the pressure side was counted. The expression of cathepsin K change of periodontal tissue was detected by Western blot and expression changes of high expression microRNA in periodontal tissue were detected through real-time Quantitative PCR, such as miR-17, miR-21, miR-126, miR-32, miR-147, miR-155.Result： Compared with the control group, HE staining results showed that periodontal tissue morphogenesis of the orthodontic group had obvious pathology change, and the osteoclast number of the pressure side was significantly higher than that of the control group （P〈0.05） .In addition, the orthodontic group also showed obviously up-regulated the expression of cathepsin K, and was accompanied by expression turbulence of miR-17, miR-21, miR-126, miR-32, miR-147, miR- 155 in the periodontal tissue （ P〈0.05 ） .While in the minimally invasive group, the configuration of periodontal tissue, osteoclast number of pressure side, the expression of

关 键 词：超声微创外科技术 正畸治疗 组织蛋白酶K MICRORNA 

分 类 号：R783.5[医药卫生—口腔医学]