UPLC法同时测定香椿芽中8种黄酮醇苷  

Simultaneous determination of eight flavonol glycosides in Toona sinensis shoots by UPLC

作　　者：葛重宇[1] 林玲[2] 顾芹英 李楠 杨欢[2] 贾晓斌[2,4] 

机构地区：[1]无锡市第三人民医院药学部,江苏无锡214000 [2]江苏大学药学院,江苏镇江212013 [3]镇江市药品检验所,江苏镇江212050 [4]江苏省中医药研究院,江苏南京210028

出　　处：《中成药》2017年第9期1873-1876,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81303313);江苏省自然科学基金(BK20150463);江苏省"六大人才高峰"项目(YY-010);江苏省药学会-奥赛康医院药学基金(A201619);江苏大学学生科研立项(14A145)

摘　　要：目的建立UPLC法同时测定香椿Toona sinensis Roemer芽中芸香苷、杨梅苷、金丝桃苷、异槲皮苷、番石榴苷、紫云英苷、槲皮苷、阿福豆苷的含有量。方法香椿芽60%甲醇提取液的分析采用Waters XBridge Shield RP18色谱柱(2.1 mm×150 mm,1.7μm);以乙腈-水为流动相,梯度洗脱;体积流量0.35 m L/min;检测波长350 nm;柱温35℃。结果 8种黄酮醇苷在各自范围内线性关系良好(r≥0.999 2),平均加样回收率98.3%~103.5%,RSD0.46%~3.36%。结论该方法准确稳定,重复性好,可用于香椿芽的质量控制。AIM To establish a UPLC method for the simultaneous content determination of glycosides,rutinoside,myricitrin,hyperoside,isoquercitrin,guaijaverin,astragalin,quercitrin and afzelin in the shoots of Toona sinensis Roemer. METHODS The analysis of 60% methanol extract of T. sinensis shoots was performed on a35 ℃ thermostatic Waters XBridge Shield RP18 column（ 2. 1 mm × 150 mm,1. 7 μm）,with the mobile phase comprising of acetonitrile-water flowing at 0. 35 m L/min in a gradient elution manner,and the detection wavelength was set at 350 nm. RESULTS Eight flavonol glycosides showed good linear relationships within their own ranges（ r ≥ 0. 999 2）,whose average recoveries were 98. 3%-103. 5% with the RSDs of 0. 46%-3. 36%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T. sinensis shoots.

关 键 词：香椿 芽 黄酮醇苷 UPLC 

分 类 号：R284.1[医药卫生—中药学]