UPLC法测定六神曲发酵过程中噻嗪双酮苷及噻嗪二酮含量  

Determination of Xanthiside and Xanthiazone in the Fermentation Process of Massa Medicata Fermentata by UPLC

作　　者：王丹[1] 胡波[1] 刘玉华[1] 王延年[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016

出　　处：《现代中药研究与实践》2017年第3期54-57,共4页Research and Practice on Chinese Medicines

基　　金：国家中医药管理局中医药行业专项"六神曲等7种中药发酵技术及规范化应用研究"(201507004-03);辽宁省教育厅科学技术研究项目:曲类中药六神曲发酵炮制机理及其新型饮片的研究(L2013387)

摘　　要：目的建立UPLC法测定六神曲发酵过程中噻嗪双酮苷及噻嗪二酮的含量测定方法,监测六神曲的发酵过程、控制六神曲的质量。方法采用UPLC法测定不同发酵天数六神曲样品中噻嗪双酮苷及噻嗪二酮的含量。结果六神曲发酵过程中噻嗪双酮苷含量降低,而噻嗪二酮的含量呈先升高后降低的趋势。噻嗪双酮苷和噻嗪二酮分别在1.40~140μg/ml(r=0.999 1)和1.19~119μg/ml(r=0.999 1)内与峰面积成良好线性关系,平均回收率分别为99.5%和101.6%。结论 UPLC法作为测定六神曲发酵过程中噻嗪双酮苷及噻嗪二酮含量的一种手段,方法简便准确、重现性和稳定性较好,适于作为控制六神曲质量的方法,为六神曲的质量评价和控制提供了科学依据。Objective To establish a method for determining the contents of Xanthiside and Xanthiazone in the fermentation process to control the quality of Massa Medicata Fermentata（ MMF）by ultra performanceliquid chromatography（ UPLC）. Methods Contents of the Xanthiside and Xanthiazone was measured by UPLC, which were in the fermentation process of MMF for seven days. Results It was showed that the content of xanthiside was reduced, but the content of xanthiazone was first increased and then decreased. The linear ranges were 1.40 to 140 μg/ml（ r = 0.999 1）, 1.19 to 119 μg/ml（ r = 0.999 1）, respectively. The average recovery were 99.5 %（ RSD = 1.6） for xanthiside and 101.6 %（ RSD = 1.9）（ n = 6） for xanthiazone, respectively. Conclusion The UPLC method was not only simple with good accuracy and reproductivity, but also could be applied for quality evaluation and control for Massa Medicata Fermentata.

关 键 词：六神曲 发酵 噻嗪双酮苷 噻嗪二酮 超高效液相 含量测定 

分 类 号：R284[医药卫生—中药学]