幽门螺杆菌在两种液体培养基中生长规律的探讨  

Characteristics of Helicobacter pylori growth in two liquid media

作　　者：荣倩玉 李娇娇[1] 季晓飞[1] 张莹[1] 耿丽[1] 李波清[1] 

机构地区：[1]滨州医学院病原生物学教研室,山东烟台264003

出　　处：《中国病原生物学杂志》2017年第8期738-740,共3页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.81471561);滨州医学院青年骨干教师培养计划经费资助

摘　　要：目的分别用血清脑心浸液和β-环糊精脑心浸液两种培养基培养幽门螺杆菌,观察幽门螺杆菌在两种培养基中的生长规律。方法将幽门螺杆菌分别接种于血清脑心浸液和β-环糊精脑心浸液中,置37℃微需氧环境以120r/min震摇培养,于不同时间点以稀释平板计数法计数活菌数量,制作生长曲线。结果幽门螺杆菌在血清脑心浸液和β-环糊精脑心浸液中的迟缓期均为10h,对数期分别为45h和40h,对数中期时比生长速率分别为0.058/h和0.043/h,稳定期分别为15h和20h,最高浓度分别为2.067×10~8 CFU/ml和1.378×10~8 CFU/ml。结论幽门螺杆菌在血清脑心浸液和β-环糊精脑心浸液中均生长良好,其中在血清脑心浸液中最高生长密度更大,对数期维持时间较长。Objectives To culture Helicobacter pylori in a serum and brain heart infusion broth and in aβ-cyclodextrin and brain heart infusion broth and to observe the growth of H.pylori in these two types of culture media Methods H.pylori was inoculated in the serum and brain heart infusion broth and in theβ-cyclodextrin and brain heart infusion broth and shaken at a speed of 120r/min in 37 ℃ microaerophilic environment.Live bacteria were counted using dilution plate counting,and a growth curve was generated. Results The lag phase of H.pylori in these two types of culture media was 10 hours.The log phase of H.pylori in the serum and brain heart infusion broth was 45 hours while that in theβ-cyclodextrin and brain heart infusion broth was 40 hours.The specific growth rate was respectively 0.058/h and 0.043/h in the mid-log phase.The stationary phase was 15 hours and 20 hours,respectively.The highest concentration of H.pylori was 2.067×10~8 CFU/ml and 1.378×10~8 CFU/ml,respectively. Conclusion H.pylori grew well in the serum and brain heart infusion broth and in theβ-cyclodextrin and brain heart infusion broth.However,H.pylori cultured in the serum and brain heart infusion broth grew at a higher density and had a longer log phase.

关 键 词：幽门螺杆菌 Β-环糊精 血清 脑心浸液 

分 类 号：R378.99[医药卫生—病原生物学]