羊源沙门氏菌耐药性和耐药基因的检测  

Detection of the antibiotic resistance and related resistance genes of Salmonella

作　　者：江萍[1] 关茹飞 夏利宁[1] 徐志勇[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《中国农业大学学报》2017年第9期55-63,共9页Journal of China Agricultural University

基　　金：国家自然科学基金-新疆联合基金项目(U1503185)

摘　　要：为掌握新疆某养殖场羊源沙门氏菌对常用抗菌药物的耐药情况,并了解其携带部分相关耐药基因的流行现状及β-内酰胺酶、16SrRNA甲基化酶和喹诺酮类耐药基因(Plasmid mediated quinolone resistance,PMQR)的共存情况。使用琼脂稀释法对分离的沙门氏菌进行药敏试验,通过PCR方法进行blaTEM、blaCMY-2、blaCTX-M、blaLAP-1、blaKPC、blaOXA、blaSHV等β-内酰胺酶和armA、rmtB等16SrRNA甲基化酶及qnrA、qnrB、qnrC、qnrD、qnrS、qepA、oqxA、oqxB和aac(6′)-Ib-cr等PMQR基因检测,分析阳性菌株携带的基因型与耐药表型之间的关系。结果显示新疆该羊养殖场分离的沙门氏菌对被检的12种抗菌药物耐药率超过80%的有6种。47株菌携带blaTEM基因,34株菌携带blaOXA基因;未检出armA和rmtB等16SrRNA甲基化酶。33株菌携带qnrS,46株菌携带oqxA,46株菌携带oqxB,47株菌携带acc(6′)-Ib-cr基因。新疆该养殖羊场分离株耐药现象严重,携带耐药基因以blaTEM、blaOXA、qnrS、aac(6′)-Ib-cr、oqxA和oqxB为主,耐药基因型复杂多变、呈多样化发展,应该加强对β-内酰胺酶及PMQR因子的监控。In order to understand the antibiotic resistance and epidemic status of some resistance genes of Salmonella isolated from a sheep farm in Xinjiang,and investigate their coexistences with β-lactamases,16S rRNA methylation enzyme genes and PMQR,the minimum inhibitory concentrations of Salmonella were determined by agar dilution method.Polymerase chain reaction （PCR） was used to detect β -lactamases including blaTEM,blaCMY-2,blaCTX-M,blaLAP-1,blaKPC,blaOXA and blaSHV,16S rRNA methylation enzyme genes （armA and rmtB） and PMQRs （qnrA,qnrB,qnrC,qnrD,qnrS,qepA,oqxA,oqxB and aac（6＇）-Ib）.The relationship between the genotypes and resistance phenotypes of positive strains were analyzed.The results showed that the resistance rate of Salmonella to 6 kinds of antimicrobial drugs was more than 80%.It was found that a total of 47 strains carrying blaTEM gene,34 strains carrying the gene blaOXA,no 16S rRNA methylase armA and rmtB detected,33 strains carrying qnrS,46 strains carrying oqxA,46 strains carrying oqxB,and 47 strains carrying acc（6＇）-Ib-cr genes.Strains isolated from the sheep farm displayed high resistance to antimicrobial drugs in Xinjiang.blaTEM,blaOXA,qnrS, aac（6＇）-Ib-cr oqxA and oqxB were mainly detected resistance genes in Salmonella.And resistant genotypes were also diversifying and complex suggesting that monitoring β -lactamase enzymes and PMQR factors should be strengthened.

关 键 词：沙门氏菌 羊 抗菌药物 耐药基因 

分 类 号：S852.61[农业科学—基础兽医学]