FISH法对肺腺癌穿刺标本EML4-ALK融合基因的检测  

Detection of EML4-ALK Fusion Gene in Lung Adenocarcinoma Specimens By FISH

作　　者：成克伦[1] 周永松[1] 陈秀婵[1] 熊云刚[1] 邹阳强[1] 姜森[1] 邓刘丹 

机构地区：[1]贵州航天医院病理科,贵州遵义563000 [2]广州安必平医药科技股份有限公司技术部,广东广州510663

出　　处：《哈尔滨医药》2017年第4期314-316,共3页Harbin Medical Journal

基　　金：中国航天科工集团公司医疗卫生科研项目(2014-LCLX-009)

摘　　要：目的探讨采用肺腺癌穿刺活检标本检测棘皮动物微管相关类蛋白4(EML4)与间变性淋巴瘤激酶(ALK)融合基因(EML4-ALK)的可行性。方法对20例肺腺癌经皮肺穿刺标本应用荧光原位杂交(FISH)法检测EML4-ALK融合基因。结果20例标本中,19例通过FISH检测成功,检测成功率为95.00%。其中阳性2例,阳性率为10.00%。结论肺腺癌穿刺活检标本可用作检测EML4-ALK融合基因,为靶向治疗提供参考依据。Objective To test the feasibility of detecting echinoderm microtubule related protein 4 （EML4）and anaplastic lymphoma kinase （ALK）fusion gene （EMIA-ALK）in lung adenocarcinoma biopsy samples.Methods Fluorescence in situ hy- bridization（FISH）was performed for 20 lung adenocarcinoma biopsy samples to detect EMIA-ALK fusion gene.Results FISH assay was successful for 19 of the 20 samples,with a success rate of 95%.2 of the 20 cases （10%）was positive for EML4-ALK fusion gene.Conclusion Lung adenocarcinoma biopsy specimens can be used for detection of EML4-ALK fusion gene,which provides ref- erences for targeted therapy of this disease.

关 键 词：荧光原位杂交 肺腺癌 EML4-ALK融合基因 经皮肺穿刺 

分 类 号：R734.2[医药卫生—肿瘤]