ω-3PUFAs Prevent MK-801-induced Cognitive Impairment in Schizophrenic Rats via the CREB/BDNF/TrkB Pathway  

作　　者：房茂胜 李行 钱红 曾宽 叶萌 周勇杰 李辉 王小川 李毅 

机构地区：[1]Wuhan Mental Health Center,Wuhan 430022,China [2]Department of Pathophysiology,School of Basic Medicine and the Collaborative Innovation Center for Brain Science,Key Laboratory of Ministry of Education of China for Neurological Disorders,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [3]Co-innovation Center of Neuroregeneration,Nantong 226001,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2017年第4期491-495,共5页华中科技大学学报（医学英德文版）

基　　金：supported in parts by grants from the Hubei Province Key Technology R&D Program(No.2015BCE094);Wuhan Science and Technology Bureau Dawn Plan Project(No.201507040410216);Clinical Research Physician Program of Tongji Medical College;HUST and the Academic Frontier Youth Team Project of HUST

摘　　要：This study was to determine the protective effect of ω-3 polyunsaturated fatty acids（ω-3PUFAs） on MK-801-induced cognitive impairment in schizophrenia（SZ） rats and the underlying mechanism. A rat model of schizophrenia was induced by MK-801. The cognitive function of rats was assessed using a Morris water maze. The number of hippocampal neurons was measured by Nissl staining. The expression of CREB, p-CREB, BDNF, TrkB, p-TrkB, AKT, p-AKT, ERK, and p-ERK in the hippocampus of rats was detected by Western blotting. The results showed that ω-3PUFAs attenuated MK-801-induced cognitive impairment and hippocampal neurons loss, reversed the injury of the CREB/BDNF/TrkB pathway induced by MK-801, and antagonized MK-801-induced down-regulation of p-AKT and p-ERK in the hippocampus of rats. In conclusion, ω-3PUFAs enhances the CREB/BDNF/TrkB pathway by activating ERK and AKT, thereby increasing the synaptic plasticity and decreasing neuron loss, and antagonizing MK-801-induced cognitive impairment in schizophrenic rats.This study was to determine the protective effect of ω-3 polyunsaturated fatty acids（ω-3PUFAs） on MK-801-induced cognitive impairment in schizophrenia（SZ） rats and the underlying mechanism. A rat model of schizophrenia was induced by MK-801. The cognitive function of rats was assessed using a Morris water maze. The number of hippocampal neurons was measured by Nissl staining. The expression of CREB, p-CREB, BDNF, TrkB, p-TrkB, AKT, p-AKT, ERK, and p-ERK in the hippocampus of rats was detected by Western blotting. The results showed that ω-3PUFAs attenuated MK-801-induced cognitive impairment and hippocampal neurons loss, reversed the injury of the CREB/BDNF/TrkB pathway induced by MK-801, and antagonized MK-801-induced down-regulation of p-AKT and p-ERK in the hippocampus of rats. In conclusion, ω-3PUFAs enhances the CREB/BDNF/TrkB pathway by activating ERK and AKT, thereby increasing the synaptic plasticity and decreasing neuron loss, and antagonizing MK-801-induced cognitive impairment in schizophrenic rats.

关 键 词：schizophrenia MK-801 ω-3 polyunsaturated fatty acids cognition impairment CREB/BDNF/TrkB pathway 

分 类 号：R749.3[医药卫生—神经病学与精神病学]