纳米囊制剂对白血病多药耐药细胞影响的体外实验研究  

In vitro experimental study of effects of nanocapsules on multidrug resistance of leukemia cells

作　　者：吴平平[1] 

机构地区：[1]江苏省肿瘤医院肿瘤内科,210009

出　　处：《中国实用医药》2017年第23期3-5,共3页China Practical Medicine

摘　　要：目的探讨纳米囊制剂对白血病多药耐药细胞的影响。方法采用溶剂挥发法制备共载中药甘草酸(GA)及化疗药物柔红霉素(DNR)的纳米囊,将其分为P-gp Ab修饰共载中药GA及化疗药物DNR组(A组);P-gp Ab修饰装载DNR化疗药物组(B组);DNR化疗药物组(C组);DNR+GA组(D组);P-gp Ab修饰纳米囊空载组(E组);GA组(F组);生理盐水对照组(G组)。采用体外四甲基偶氮唑盐(MTT)比色法评价细胞毒作用(白血病多药耐药细胞K562/A02),采用流式细胞仪检测细胞凋亡的情况,采用化学发光自显影免疫印迹法(Western Blot)检测蛋白B淋巴细胞瘤-2相关X蛋白(Bax)、B淋巴细胞瘤-2(Bcl-2)的表达。结果 A组的K562/A02的半抑制浓度指数(IC_(50))为(3.65±1.06),其降低效果优于其他组(P<0.05)。B组IC_(50)为(3.12±0.96),低于C、D、F组的(0.95±0.04)、(0.96±0.03)、(0.94±0.05)(P<0.05)。C、D、F组的耐药指数(RE)均接近1,故认为对K562/A02的细胞无增强耐药作用,也无毒性作用。E组和G组为对照组,检测到的细胞掉凋亡率较小,不计入比较。A组的细胞总凋亡率高于其他各组(P<0.05)。B组的细胞凋亡率高于C、D、F组(P<0.05)。A组的Bcl-2的表达低于其他各组,Bax的表达高于其他各组(P<0.05)。E组和G组的Bcl-2表达均高于其他各组,Bax的表达均低于其他各组,差异均具有统计学意义(P<0.05)。结论纳米囊制剂对白血病多药耐药细胞具有细胞毒性,中药GA+化疗药物DNR具有明显的协同效果,其作用机制可能与上调Bax表达与下调Bcl-2有关。Objective To investigate the effect of nanocapsules on multidrug resistance of leukemia cells. Methods Nanocapsules containing Chinese medicine glycyrrhizin（GA） and chemotherapy drug daunorubicin（DNR） were prepared by solvent evaporation method. They were divided into P-gp Ab modified loading traditional Chinese medicine GA and chemotherapy drug DNR group（group A） and P-gp Ab modified loading DNR chemotherapy group（group B）, DNR chemotherapy drug group（group C）, DNR＋GA group（group D）, P-gp Ab modified nanocapsules empty group（group E）, GA group（group F） and saline control group（group G）. The cytotoxicity（leukemia multidrug resistance cell K562/A02） was evaluated by methylthio tetrazole（MTT） colorimetric assay, apoptosis was detected by flow cytometry, and the expression of B-cellymphoma-2 associated X protein（Bax） and B-cellymphoma-2（Bcl-2） were detected by chemiluminescence autoradiography（Western Blot）. Results Group A had semi inhibitory concentration index（IC_（50）） of K562/A02 as（3.65±1.06）, and its reduction effect was better than the other groups（P〈0.05）. Group B had IC_（50） as（3.12±0.96）, which were lower than（0.95±0.04）,（0.96±0.03） and（0.94±0.05） in group C, group D and group F（P〈0.05）. The resistance factor（RI） of group C, D and F was close to 1, so it was considered that the K562/A02 cells had no enhanced drug resistance and non-toxic effect. Group E and group G as the control group, their detected cell outage rate was small, so they were not included in the comparison. Group A had higher total cell apoptosis rate than the other groups（P〈0.05）. Group B had higher cell apoptosis rate than group C, group D and group F（P〈0.05）. Group A had lower expression of Bcl-2 than the other groups, and higher expression of Bax than the other groups（P〈0.05）. Group E and group G had higher expression of Bcl-2 than the other groups, and lower expression of Bax than the other groups. Their diff

关 键 词：纳米囊制剂 白细胞 多药耐药 K562/A02 体外实验 

分 类 号：R733.7[医药卫生—肿瘤]