免标记型生物传感器比色法快速检测癌胚抗原  

Ultrasensitive One-step Rapid Visual Detection of CEA by Label-free Aptasensor

作　　者：程克文 潘道东[1] 腾军 陈伟 

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]合肥工业大学食品科学与工程学院,安徽合肥230009

出　　处：《核农学报》2017年第9期1782-1787,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家支撑计划(2015BAD17B02-3)

摘　　要：为了快速的检测癌胚抗原(CEA),将胶体金与适配体室温孵育后加入CEA以及氯化钠溶液,随着CEA浓度的增加,分析胶体金溶液颜色的变化情况。结果表明,试验中含有2种不同核酸适配体(18个碱基和19个碱基)的胶体金溶液,其颜色都是随着CEA浓度增加均呈梯度变化,颜色梯度为从酒红色到深蓝色。实际样品的检测结果也呈现出良好的颜色梯度变化,CEA检测限为2 ng·mL^(-1),检测范围为0~200 ng·mL^(-1),且检测方法特异性良好。本研究结果为疾病的前期诊断提供了简便快速廉价的检测方案。In order to detect carcinoembryonic antigen （CEA） rapidly, gold nanoparticles （GNPs） and aptamer were incubated at room temperature, then added the CEA and NaC1. With the concentration increase of of CEA, the color change of GNPs was analyzed. The results showed that the color of GNPs contains two CEA aptamers （18 - met and 19 - mer） both changed with the concentration of CEA, from wine red to blue. The color changed showed the same trends when with real samples, in which the CEA concentrations ranged from 0 to 200 ng mL-1 and the detection limit was 2 ng mL -1 with fine specificity. The developed aptasensor may provide a simple, rapid and economic tool for early diagnosis of CEA.

关 键 词：癌胚抗原 胶体金 适配体 比色法 

分 类 号：R730.43[医药卫生—肿瘤]