柴胡解毒汤对人肝星状细胞LX-2增殖及凋亡的影响  

Influence of Chaihu Jiedu Decoction on LX-2 Cell Proliferation and Apoptosis

作　　者：王珏云 田聪阳 邹金桥[3] 赵公泽 白鑫洁 杨先照[1,2] 

机构地区：[1]北京中医药大学第一临床医学院,北京100700 [2]北京中医药大学东直门医院,北京100700 [3]北京中医药大学中医学院,北京102446 [4]北京中医药大学针灸推拿学院,北京100029

出　　处：《现代生物医学进展》2017年第24期4610-4614,4625,共6页Progress in Modern Biomedicine

基　　金：国家"十二五"科技重大专项课题(2012ZX10005010-002-005);教育部大学生创新性实验计划项目(201610026010)

摘　　要：目的:观察柴胡解毒汤含药血清对人肝星状细胞LX-2增殖、凋亡情况的影响,探究其抗肝纤维化作用的可能机制。方法:将Wistar大鼠分为实验组与对照组,分别以柴胡解毒汤、生理盐水灌胃5天后取血制备大鼠含药血清与正常血清。同时复苏人肝星状细胞LX-2后进行细胞培养和细胞传代。当肝星状细胞数量达到预定值时,将肝星状细胞分为对照组和药物组。观察LX-2细胞在与含药血清孵育24 h、36 h、48 h、72 h后,用CCK-8比色法测定细胞的增殖抑制率。用流式细胞术(Annexin V-FITC,PI染色法)检测细胞凋亡的概况。结果:柴胡解毒汤含药血清在给药24 h后可抑制LX-2细胞的增殖,36 h、48 h、72 h的增殖抑制率分别为0.37%、0.46%、0.44%,并可诱导LX-2细胞发生凋亡,48 h、72 h凋亡概率分别为(9.80±0.95)%、(36.40±5.09)%,差异具有统计学意义(P<0.05)。结论:柴胡解毒汤具有抑制LX-2细胞增殖、诱导LX-2细胞发生凋亡的能力,这可能是柴胡解毒汤抗纤维化作用的机制之一。Objective：To observe the effects of Chaihu Jiedu decoction on human hepatic stellate LX-2 cells,and to explore the potential molecular mechanisms.Methods：The wistar rats were divided into the experimental group and the control group,respectively with Chaihu Jiedudecoction and saline lavage,then centrifugal and get the drug-containing serum and control serum.At the same time,trsuscitate cells.When we got the expected number of cells,we divided into the experimental group and the control group.The human hepatic stellate cells （LX-2） were with the drug serum for 24 h,36 h,48 h,72 h.Then the cell proliferation inhibition rate was measured by CCK-8,the apoptosis were detected by flow cytometry （Annexin V-FITC,PI staining method）.Results：ChaihuJiedu decoction could inhibit proliferation of LX-2 cells 24 hours after dosing.The in hibition rate in 36 h,48 h,72 h were 0.37 ％,0.46 ％,0.44 ％ respectively.It could prevent LX-2 cells into proliferation and induce the apoptosis of LX-2 cells.The apoptosis rates in 48 h,72 h were （9.80±0.95）％,（36.40± 5.09）％ respectively,and there are difference of statistical significance（P＜0.05）.Conclusion：Chaihu Jiedu decoction can inhibit the proliferation of LX-2 cells proliferation,and induce apoptosis,so as to interfere with course of the liver fibrosis.

关 键 词：柴胡解毒汤 人肝星状细胞 增殖 凋亡 细胞实验 

分 类 号：R-33[医药卫生] R575.2