出生早期母婴分离对新生小鼠神经元细胞凋亡的影响  

Effect of Early Mother-to-child Isolation on Neuronal Cell Apoptosis in Neonatal Mice

作　　者：杨海鹏[1] 王利微 邵庆亮[1] 高越[1] 赵璇[1] 曲学华[1] 

机构地区：[1]哈尔滨医科大学附属第四医院新生儿科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2017年第26期5034-5037,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科研项目(12541286)

摘　　要：目的:探讨出生早期母婴分离对新生儿神经系统的影响及其相关机制。方法:随机选择2015年1月~2015年9月出生的新生小鼠120例作为本次研究的对象,其中新生小鼠出生后实施母婴分离的作为观察组(60只),出生后不实施母婴分离的作为对照组(60只)。比较两组新生小鼠的神经系统、神经细胞的变化。结果:观察组新生小鼠在母婴分离第7天、14天、21天的神经元细胞的凋亡率显著高于对照组新生(P值均<0.05),神经元胱天蛋白酶-3(Caspase-3)蛋白表达均明显高于对照组(P值均<0.05)。观察组新生小鼠的母婴分离14天、21天的神经胶质细胞小窝蛋白-1(Caveo-1)蛋白的表达,与对照组新生小鼠的神经胶质细胞Caveo-1蛋白的表达进行比较,(P值均<0.05),具有统计学意义。结论:出生早期实施母婴分离,对新生儿的神经系统会产生较大的影响,影响新生儿发育中神经系统的表达,进而会影响新生儿成年后的行为发育异常。Objective： To explore the effect of early mother to child separation on neonatal nervous system and its related mechanism. Methods： Randomly selected during January 2015 to September 2015, was born in 120 cases of newborn mice as the research object, the newborn mice was born after the implemented of mother-to-child separation as observation group （60）, not the implemented of separation of mother and baby after birth as the control group （60）, in view of the two groups of newborn mice of the nervous system, the change of the nerve cells were compared and researched. Results： The apoptotie rate of neurons in the neonatal mice was significantly higher than that in the control group （P 〈0.05）. The neurons ofcaspase- 3 protein expression was significantly higher than the control group （P 〈0.05）. The expression of Caveo-1 protein in the glial cells of 14 days and 21 days was observed in the neonatal mice. The expression of Caveo-1 protein in the glial cells of the control group For comparison, （P 〈0.05）, which were statistically significant. Conclusion： The early implementation of maternal and infant birth separation of neonatal nervous system will produce great influence, and influence the expression of the nervous system in the development of newborn, which affect the behavior of the neonatal adult dysplasia.

关 键 词：新生儿 母婴分离 神经系统 

分 类 号：R-33[医药卫生] R722