益气健脾方对Wistar鼠大肠癌模型中异常腺窝病灶的影响及其相关机制的探讨  

Effect of Yiqi Jianpi Fang on Aberrant Crypt Foci Production in Carcinogenesis Wistar Rat Colorectal Cancer Model and Investigation on the Related Mechanism

作　　者：赵凡尘[1] 李雁[1] 骆莹滨 周奕阳[1] 徐夏婷[1] 

机构地区：[1]上海中医药大学附属市中医医院肿瘤科,上海200071

出　　处：《现代生物医学进展》2017年第26期5043-5047,共5页Progress in Modern Biomedicine

基　　金：上海中医药大学科技计划项目(2014YSN58)

摘　　要：目的:探讨益气健脾方对大肠癌模型异常腺窝病灶(ACF)的抑制效应及其相关机制。方法:50只Wistar大鼠随机分为5组:中药低剂量组(将中药溶液稀释3倍后,5 mL/kg日灌胃量)、中药中剂量组(5 mL/kg日灌胃量)、中药高剂量组(15 m L/kg日灌胃量)、模型组、正常组,每组10只大鼠,采用免疫组化法、美兰染色后镜下观察大肠组织。结果:各中药组ACF及大型ACF的数量较模型组减少(P<0.05),并且中药中剂量组ACF数量减少最为明显,差异具有统计学意义(P<0.05)。造模后24h、48h中药组肠腺细胞PCNA增殖指数低于模型组,差异具有统计学意义(P<0.05)。造模后24 h、48 h中药组肠腺细胞凋亡指数高于模型组,差异具有统计学意义(P<0.05)。中药组β-catenin异位表达均低于模型组,并且中药组β-catenin异位表达中高剂量组最高,低剂量组次之,中剂量组最低(P<0.05)。中药组MMP-7的表达均低于模型组,而且各中药组中低剂量MMP-7表达最高,高剂量组次之,中剂量组最低(P<0.05)。结论:益气健脾方可以明显降低Wistar大鼠ACF的发生数量,抑制大肠癌发生中Wnt信号的激活,在一定程度上减少了大肠癌的发生,起到了预防作用。Objective： To investigate the inhibitory effect and the related mechanism of Yiqi Jianpi Fang on aberrant crypt focus （ACF） in Wister rat colorectal cancer model. Methods： 50 Wistar rats were randomly divided into 5 groups： TCM low dose group（TCM solution diluted 3 times, 5 mL/kg daily gastric volume）, TCM middle dose group （5 mL/kg daily gastric volume）, TCM high dose group （15 mL/kg daily gastric volume）, model group, normal group. With 10 rats in each group. The coloreetal tissues were observed under microscope after methylene blue staining by immunohistoehemistry method. Results： Compared with the model group, the number of ACF and large ACF in each TCM group were decreased （P〈0.05）, and the number of ACF in the TCM middle dose group reduced most obvious, and the difference was statistically significant （P〈0.05）. The proliferation indexs of PCNA in intestinal gland cells 24 h and 48 h after modeling in the TCM groups were higher than those in the model group, and the difference was statistically significant（P〈0.05）. The apoptosis index of intestinal gland cells 24 h and 48 h after modeling in the TCM groups were higher than those in the model group, and the difference was statistically significant（P〈0.05）. The ectopic expression of β-catenin in TCM groups were lower than that in the model group, and it was highest in the high dose group, than was the low dose group, and the middle group was lowest（P〈0.05）. The expression of MMP-7 in TCM groups were lower than that in the model group,and it was highest in the low dose group, than was the high dose group, and the middle dose group was lowest （P〈0.05）. Conclusion： Yiqi Jianpi Fang can significantly reduce the number of ACF in Wister rats, inhibit the activation of Wnt signaling in eolorectal cancer,reduce the incidence of colorectal cancer, and have a certain preventive effect.

关 键 词：大肠癌 异常腺窝病灶 益气健脾 

分 类 号：R-33[医药卫生] R735.34