饲料中添加干酪乳杆菌对鲤鱼非特异性免疫力和抗病力的影响  

Effects of non-specific immunity and disease resistance of feeding Lactobacillus casei on common carp

作　　者：张海朋 谷巍[2] 鞠安琪 毕建飞[1] 曲磊[1] 成璐 陈龙[1] 康元环[1] 单晓枫[1] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]山东宝来利来生物工程股份有限公司,山东泰安271000

出　　处：《中国兽医杂志》2017年第7期85-88,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(31201927);吉林省重点科技攻关项目(20150204065NY)

摘　　要：为探讨饲料中添加干酪乳杆菌对非特异性免疫力和抗病力的影响,在基础饲料中分别添加3种不同浓度1×107CFU/g、1×108CFU/g、1×109CFU/g的干酪乳杆菌,养殖初始体重为(28±0.3)g的鲤鱼6周。结果显示,与对照组相比,饲料中添加1×108CFU/g时超氧化物歧化酶(SOD)和抗体Ig M含量显著升高(P<0.05),但1×109CFU/g的SOD活力和抗体Ig M含量反而受抑制;在28 d时添加量为1×108CFU/g的溶菌酶(LZM)和过氧化物酶(POD)活力最高,1×107CFU/g、1×109CFU/g的低一些,均高于对照组;而试验各组中碱性磷酸酶(AKP)活力随干酪乳杆菌浓度的增加而升高。饲喂后第42天以菌株维氏气单胞菌TH0426攻毒,各组14 d后的累积死亡率差异显著(P<0.05),与对照组相比,添加1×108CFU/g的干酪乳杆菌免疫保护率最高为60%,1×107CFU/g、1×109CFU/g的次之。由此可见,在基础饲料中添加1×108CFU/g的干酪乳杆菌能提高鲤鱼的非特异性免疫力,且具有较高的免疫保护效果。To investigate the effects of the feed of Lactobacillus casei on non - specific immunity and disease resistance in carp, three different concentrations of Lactobacillus easel （ 1 × 107 CFU/g, 1 ×108 CFU/g, 1 × 109 CFU/g） were added to the basal diet for 6 weeks, and the initial culture weight of carp was 28 ± 0.3g. The results showed that the contents of SOD and IgM increased signifi- cantly （P 〈 0.05） at 1 × 108CFU / g, but the contents of SOD and IgM were inhibited at 1 ×109CFU/g. The LZM and POD ac- tivities （1 ×108CFU/g） were the highest at28d, andl x 107CFU/gand 1 ×109CFU/gwaslowerthanl x IOsCFU/g, all the experimental groups were higher than the control group. While the activity of AKP in all experimental groups increased with the concentration of Laetobacillus casei. On the 42 day after feeding, the fishes were challenged with Aeromonas hydrophila strain TH0426, there were significant differences in the cumulative mortality after 14 days （ P 〈 0.05 ）. Compared with the control group, the immune protection rate of Lactobacillus with 1 × 108CFU/g was 60%, followed by 1 × 107 CFU/g, 1 × 109CFU/g. Thus, adding 1 ×108 CFU / g of Lactobacillus casei to the basal diet could improve the non - specific immunity of carp and have higher immune protection effect.

关 键 词：干酪乳杆菌 鲤鱼 酶活力 免疫效果 

分 类 号：TS2[轻工技术与工程—食品科学与工程]