桦褶孔菌多糖的分离纯化及清除自由基活性  

Isolation, purification and free radical scavenging activities of polysaccharide from Lenzites betulina

作　　者：申翔宇[1,2] 马涛 马希 黄雅婷 刘春兰[1,2] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081 [2]中央民族大学北京市食品环境与健康工程技术研究中心,北京100081

出　　处：《菌物学报》2017年第9期1271-1277,共7页Mycosystema

基　　金：中央民族大学一流大学一流学科建设项目(YLDX01013);"111创新引智计划"工程项(B08044);准格尔北部生态保育技术集成与示范(2014BAC15B04);2017年统筹推进一流大学和一流学科建设经费;中央民族大学学术团队建设项目(2015MDTD25C&13C)~~

摘　　要：利用水提醇沉法对桦褶孔菌Lenzites betulina粗多糖HZKJc进行提取,随后经Sevage法和酶法联合脱蛋白,再经DEAE‐Sephadex A‐25,G‐100柱层析纯化后得到多糖纯品HZKJv。通过高效液相色谱(HPLC)测定上述纯化多糖的纯度和相对分子量分布;经纸层析(PC)、气相色谱(GC)、红外光谱(IR)进行单糖组成分析;并研究其清除自由基活性。分离纯化后得到的HZKJv为纯多糖,相对分子质量约为11 687。当HZKJv溶液浓度为3.0mg/m L时,其DPPH自由基清除率可达82%;浓度为2.0mg/m L时,对?OH自由基的清除率可达85%。表明HZKJv对?OH与DPPH自由基存在很好的清除作用。The crude polysaccharide named as HZKJc was extracted from Lenzites betulina by water extraction and alcohol precipitation, and then deproteinized by Enzyme-Sevage combined method. The crude polysaccharide was purified by DEAE-Sephadex A-25 and G-100 gel filtration chromatography to obtain pure polysaccharide products HZKJv. The purity and relative molecular weight distribution of HZKJv were determined by high performance liquid chromatography （HPLC）. Monosaccharide composition was analyzed by paper chromatography （PC）, gas chromatography （GC） and infrared spectroscopy （IR）. The free radical scavenging activities of purified polysaccharide were investigated. HZKJv was a homogeneous polysaccharide with average molecular concentration of HZKJv was 3.0mg/mL and 2.0mg/mL, the .OH and DPPH free radical scavenging rate respectively, indicating that HZK_Iv had excellent scavenging effect on .OH and DPPH free radicals weight of 11 687. When the can be up to 82% and 85%.

关 键 词：桦褶孔菌多糖 HPLC 单糖组成 抗氧化活性 

分 类 号：R284[医药卫生—中药学]