红树植物老鼠簕内生细菌数量的时空动态变化  

Spatiotemporal dynamic of endophytic bacteria numbers in Acanthus illicifolius

作　　者：林巧玲[1,2] 严玉宁 何红[1] 孙兴涛[1] 杨定祥[1] 

机构地区：[1]广东海洋大学农学院,广东湛江524088 [2]省级现代农业(热带特色园艺)产业技术研发中心,广东湛江524088

出　　处：《微生物学通报》2017年第9期2037-2042,共6页Microbiology China

基　　金：广东省科技计划项目(No.2015A020209126)~~

摘　　要：【目的】了解红树植物老鼠簕内生细菌数量的时空动态变化。【方法】分别用荧光显微计数法和平板计数法对老鼠簕内生细菌总量和可培养内生细菌总量进行周年动态测定。【结果】老鼠簕根、茎、叶部内生细菌总量年平均分别为12.25×107、7.59×107和5.72×107 ind./g(FW);而可培养内生细菌总量年平均分别为3.98×103、3.70×103和2.18×103 CFU/g(FW)。【结论】老鼠簕植株体内含有大量的内生细菌,根部含量最高,茎部次之,叶部最少。但分离的可培养内生细菌占比极少(仅为0.000 2%-0.146 8%)。老鼠簕内生细菌总量及可培养内生细菌总量均随采样季节不同而呈现波动性变化,内生细菌总量最大值出现在夏季(7月),可培养内生细菌总量最大值出现在春季(3月)。[Objective] To study the spatiotemporal dynamic of endophytic bacteria numbers in Acanthus illicifolius. [Methods] Annual dynamic of total endophytic bacteria and culturable endophytic bacteria in different organs ofA. illicifolius were studied using fluorescence microscopy and plate counting method, respectively. [Results] The number of total endophytic bacteria in roots, stems and leaves was 12.25× 107, 7.59× 107 and 5.72× 107 ind/g（FW） successively. And the number of culturable endophytic bacteria corresponds to 3.98×103, 3.70×103 and 2.18×103 CFU/g（FW）. [Conclusion[ There were numerous endophytic bacteria in A. illicifolius. The highest concentration of endophytic bacteria was in roots, followed by stems and leaves. Culturable endophytic bacteria accounted for only 0.000 2%-0.146 8% of total endophytic bacteria in A. illicifolius. The fluctuation

关 键 词：老鼠簕 内生细菌 时空动态 

分 类 号：Q93[生物学—微生物学]