VX2兔肝门静脉主干癌栓模型建立的实验研究  

Establishing rabbit model of main portal vein VX2 tumor thrombus

作　　者：任葆胜[1,2] 杨正强 施海彬[1] 刘圣[1] 

机构地区：[1]南京医科大学第一附属医院介入放射科,江苏南京210029 [2]南京医科大学附属常州第二人民医院介入血管科,江苏常州213161

出　　处：《实用放射学杂志》2017年第9期1454-1457,共4页Journal of Practical Radiology

基　　金：江苏省卫生厅开放课题（RC52200901）.

摘　　要：目的 利用原位种植的方法制备VX2兔肝门静脉主干癌栓动物模型，观察癌栓生长过程中的影像学与病理学特点。方法 新西兰大白兔24只，随机分为实验组（16只）和对照组（8只）。实验组通过外科手术方式将VX2瘤块固定于门静脉主干内壁。对照组取自体肌肉组织块以同样方法固定于门静脉主干内壁。术后分别于第7、14、21天和第28天对2组兔进行CT检查。实验组每个观察点随机取3只兔进行DSA检查，随后处死作病理学检查。对照组在每个观察点完成CT检查后随机取1只兔处死做病理检查。剩余兔观察生存时间。结果 实验组16只兔中15只顺利完成手术，接种成功率93.8%，15只兔门静脉主干内均形成癌栓，剩余3只兔平均生存时间为（39.3±2.1） d。CT及DSA检查可显示癌栓影像学特征，病理学检查证实癌栓存在并发现新生肿瘤血管形成。结论 通过原位种植的方法可以成功获得门静脉主干癌栓的动物模型，其影像和病理特征与人原发性肝癌合并的门静脉癌栓相似。Objective To establish the rabbit model of main portal vein VX2 tumor thrombus by using orthotopic implantation and to observe its radiological and pathological features.Methods 24 New Zealand white rabbits were randomly divided into study group （n=16） and control group （n=8）.In study group, VX2 tumor tissue fragments were fixed on the inner wall of the main portal vein by using a surgical approach. In control group, autologous muscle fragments were fixed on the inner wall of the main portal vein in the same way. CT examinations were performed in both group on 7 days, 14 days, 21 days and 28 days after the procedure. At each observation point, 3 rabbits of the study group were inspected by using digital subtraction angiography （DSA）, and then sacrificed for histologic examination. Meanwhile, 1 rabbit of the control group was sacrificed for histologic examination after CT scanning. The survival time of the remaining rabbits were recorded. Results Among 16 rabbits of the study group, successful implantations were achieved in 15 rabbits （ 93.8%）.Tumor thrombus formed in the main portal vein successfully for all these 15 rabbits.The mean survival time of the remaining 3 rabbits in the study group was （39.3±2.1） days. The imaging characteristic of tumor thrombus could be demonstrated by CT and DSA examinations.Tumor thrombus and the inner neovascularization were confirmed by histological examination. Conclusion It is feasible to establish an animal model of main portal vein tumor thrombus by orthotopic implantation.The radiological and pathological features of animal model are similar to hepatocellular carcinoma patients with portal vein tumor thrombus.

关 键 词：兔 门静脉 癌栓 动物模型 

分 类 号：R-332[医药卫生] R322.123