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作 者:王天媛[1] 张飞飞[1] 任跃英[1] 李嘉丰 许嘉[1] 宫丽婷[1] 吕东明[1]
出 处:《中国实验方剂学杂志》2017年第18期31-35,共5页Chinese Journal of Experimental Traditional Medical Formulae
基 金:吉林省科技型中小企业技术创新项目(sc201508007)
摘 要:目的:研究不同加工方法对猪苓药材质量的影响,为建立该药材的合理产地加工方法提供参考。方法:采用晒干、阴干、不同温度烘干共10种加工方法处理猪苓药材,参照2015年版《中国药典》中水分、折干率、总灰分、酸不溶性灰分含量测定方法测定;利用苯酚-硫酸法显色,紫外-可见分光光度法(检测波长625 nm)测定猪苓总多糖含量;运用HPLC测定麦角甾醇的含量,流动相甲醇,检测波长283 nm。结果:不同加工方法处理的猪苓药材性状差异较小,有效成分含量存在显著性差异,40℃以上烘干处理获得的猪苓折干率较大,水分含量较小;40,50℃烘干处理时总灰分和酸不溶性灰分含量较低,麦角甾醇和总多糖含量较高。结论:综合分析药材有效成分含量、生产成本等因素,猪苓产地加工方法以低温40,50℃烘干法为宜。Objective: To investigate influence of different processing methods on quality of Polyporus,and to establish its reasonable origin processing method. Method: Ten kinds of drying methods,including drying in the sun,drying in the shade,drying at different temperature,were adopted to treat Polyporus. Water content,drying rate,total ash and acid insoluble ash contents were determined according to the methods in the 2015 edition of Chinese Pharmacopoeia, phenol-sulfuric acid method was employed to measure the content of total polysaccharides at 625 nm and the content of ergosterol was determined by HPLC. Result: There was small differences in the traits of Polyporus and significant differences in the contents of active ingredients with various processing methods,the drying rate of Polyporus was large under drying at 〉40 ℃ and water content was small;under drying at 40,50 ℃, total ash and acid insoluble ash contents of Polyporus were low, the contents of ergosterol and total polysaccharides were high. Conclusion: In the comprehensive analysis of content of active components,production costs and other factors, drying at low temperature( 40,50 ℃) is the appropriate processing method for Polyporus.
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