MKP-4在肝细胞癌中的表达及临床意义  

Expression and clinical significance of MKP-4 in hepatocellular carcinoma

作　　者：刘金霞[1] 瞿利帅[1] 肖明兵[1] 江枫[1] 陆翠华[1] 倪润洲[1] 

机构地区：[1]南通大学附属医院消化内科,江苏南通226001

出　　处：《临床肿瘤学杂志》2017年第8期693-697,共5页Chinese Clinical Oncology

基　　金：国家自然科学基金青年科学基金资助项目(81401985);江苏省六大人才高峰资助项目(WSN-057)

摘　　要：目的探讨MKP-4在肝细胞癌(HCC)中的表达及其临床意义。方法收集2005年1月至2010年12月在南通大学附属医院行肝癌根治术的100例HCC切除标本及对应癌旁组织,采用免疫组化SP法检测上述组织中MKP-4的表达,并分析其与临床病理特征及预后的关系。另收集8例新鲜HCC及对应癌旁组织,采用Western blotting法半定量检测MKP-4表达。结果 MKP-4蛋白在8例新鲜HCC组织中的表达量为0.611±0.136,显著低于癌旁组织的0.931±0.107(P<0.05)。HCC组织中MKP-4的高表达率为20%,低于癌旁组织的34%,差异有统计学意义(P<0.05)。MKP-4表达与组织学分级(P=0.047)、静脉侵袭(P=0.026)和TNM分期(P=0.036)有关。Kaplan-Meier法显示,MKP-4低表达者的中位总生存时间(OS)为52个月,MKP-4高表达者的中位OS未达到,后者OS明显长于前者(P<0.05)。结论 MKP-4在HCC的发生、发展中起抑癌基因的作用,可能是HCC潜在的治疗靶点和判断预后的分子标志物。Objective To investigate the expression of MKP-4 in hepatocellular carcinoma （HCC） and its clinical signifi- cance. Methods From January 2005 to December 2010, 100 cases of HCC resection and corresponding paracancerous tissues were collected in Affiliated Hospital of Nantong University. Immunohistochemistry（ SP method） was used to detect MKP-4 expression in above tissues. Correlations between MKP-4 expression and elinicopathological parameters and prognosis were analyzed. Another 8 cases of fresh HCC and paracancerous tissues were collected, and the expression of MKP-4 was detected by Western blotting methods. Results The expression level of MKP-4 protein in 8 cases of fresh HCC tissues was 0. 611± 0. 136, which was significantly lower than 0. 931±0. 107 of paracancerous tissues（P〈0. 05）. The high expression rate of MKP-4 in HCC tissues was 20%, lower than 34% of pa- racaneerous tissues, and the difference was statistically significant （P 〈 0. 05 ）. The expression of MKP-4 was correlated with histological grade（ P = 0. 047 ） , vein invasion （ P = 0. 026 ） and TNM stage （ P = 0. 036 ）. Kaplau-Meier analysis indicated that the median overall survival of MKP-4 low expression group was 52 months, and that of high expression group was unachieved, which was longer than MKP-4 low expression group（P〈0. 05）. Conclusion MKP-4 exerts as a tumor suppressor gene in the development and progression of HCC, which suggests that MKP-4 may play as a potential therapeutic target and an efficient prognosis biomarker for HCC.

关 键 词：肝细胞癌(HCC) MKP-4 预后 

分 类 号：R735.7[医药卫生—肿瘤]