CSF-Based Analysis for Identification of Potential Serum Biomarkers of Neural Tube Defects  

作　　者：Xinyu Yan Lixin Mai Changchun Lin Wenji He Gengsheng Yin Jiakang Yu Lian Huang Sanqiang Pan 

机构地区：[1]Department of Anatomy,Medical College of Jinan University,Guangzhou 510632,China [2]Department of Anatomy,Gannan Medical University,Ganzhou 341000,China [3]Department of Clinical Laboratory,The First Affiliated Hospital of Jinan University,Guangzhou 510630,China [4]Department of Pediatric Surgery,Guangzhou Children's Hospital,Guangzhou 510623,China [5]Department of Neurology,The First Affiliated Hospital ofJinan University,Guangzhou 510630,China

出　　处：《Neuroscience Bulletin》2017年第4期436-444,共9页神经科学通报（英文版）

基　　金：supported by the Development and Innovation Foundation of Jinan University,Guangdong Province,China(21611613);the National Natural Science Foundation of China(81271280)

摘　　要：The protein composition of cerebrospinal fluid（CSF） in neural tube defects（NTDs） remains unknown. We investigated the protein composition of CSF from 9 infants with NTDs using isobaric tags for relative and absolute quantitation（iTRAQ）. We identified 568 proteins in the CSF of infants with spina bifida, which is the most common type of NTD. Among these, 18 proteins were associated with neural tube closure in the CSF during human embryonic neurulation and 5 were involved in NTDs. Based on these results, an animal model was further utilized to investigate early serum biomarkers for NTDs.We found that the myristoylated alanine-rich C-kinase substrate, Kunitz-type protease inhibitor 2, andapolipoprotein B-100 protein levels were decreased in both embryos and the sera of pregnant Sprague-Dawley rats carrying embryos with NTDs. CSF proteins may be useful in the discovery of potential serum biomarkers for NTDs.The protein composition of cerebrospinal fluid（CSF） in neural tube defects（NTDs） remains unknown. We investigated the protein composition of CSF from 9 infants with NTDs using isobaric tags for relative and absolute quantitation（iTRAQ）. We identified 568 proteins in the CSF of infants with spina bifida, which is the most common type of NTD. Among these, 18 proteins were associated with neural tube closure in the CSF during human embryonic neurulation and 5 were involved in NTDs. Based on these results, an animal model was further utilized to investigate early serum biomarkers for NTDs.We found that the myristoylated alanine-rich C-kinase substrate, Kunitz-type protease inhibitor 2, andapolipoprotein B-100 protein levels were decreased in both embryos and the sera of pregnant Sprague-Dawley rats carrying embryos with NTDs. CSF proteins may be useful in the discovery of potential serum biomarkers for NTDs.

关 键 词：Neural tube defects SERUM Cerebrospinal fluid PROTEOME Biomarkers 

分 类 号：R748[医药卫生—神经病学与精神病学]