肿瘤微环境中白细胞介素6的表达与NK/T细胞淋巴瘤预后的关系  

Relationship between expression of interleukin 6 in tumor microenvironment and prognosis in patients with natural killer/T cell lymphoma

作　　者：蔡绮纯 白冰[2] 高岩[2] 王潇潇[2] 黄慧强[2] Cai Qichun Bai Bing Gao Yan Wang Xiaoxiao Huang Huiqiang(Department of Lymphoma, Cancer Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510180, China Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China)

机构地区：[1]广东省人民医院广东省医学科学院肿瘤中心淋巴瘤科,广州510180 [2]中山大学肿瘤防治中心内科,广州510060

出　　处：《白血病．淋巴瘤》2017年第7期396-399,共4页Journal of Leukemia & Lymphoma

基　　金：国家自然科学基金（81500165）;广东省医学科学技术研究基金（201512311735141）

摘　　要：目的 探讨白细胞介素6（IL-6）在NK/T细胞淋巴瘤（NKTCL）肿瘤微环境中的表达水平及其与患者临床特征、预后的关系.方法 收集中山大学肿瘤防治中心2005年1月至2011年12月有可切取蜡块的初治NKTCL病例93例,采用免疫组织化学方法检测肿瘤微环境中IL-6的表达水平,以阳性细胞数≥10个/高倍镜视野（HP）为阳性标准.两组间计量资料、计数资料比较分别采用t检验和χ2检验,生存分析采用Kaplan-Meier法及Log-rank检验,以P〈0.05为差异有统计学意义.结果 NKTCL肿瘤微环境中IL-6表达的中位数为21个/HP（0~150个/HP）,IL-6阳性率为61.29%（57/93）.IL-6阳性率在发热、高韩国预后指数（KPI）评分患者中显著增高,差异均有统计学意义（均P〈0.05）.IL-6阳性者血清C反应蛋白水平为（29.282.62）mg/L,阴性者为（11.142.77）mg/L（t=-2.276,P=0.025）.IL-6阴性者5年无进展生存（PFS）率为52.7%,5年总生存（OS）率为60.0%;IL-6阳性者5年PFS率为23.6%,5年OS率为27.1%,两组比较差异均有统计学意义（均P=0.001）.结论NKTCL肿瘤微环境IL-6高表达与患者高危临床特征相关,IL-6高表达者长期生存差.Objective To investigate the expression of interleukin 6 （IL-6） in tumor microenvironment of natural killer/T cell lymphoma （NKTCL） and its relationship with clinical characteristics and prognosis. Methods From January 2005 to December 2012, 93 patients with NKTCL and available paraffin-embedded tissue in Sun Yat-sen University Cancer Center were included. The expression or IL-6 in tumor microenvironment was investigated by immunohistochemistry. The positive IL-6 expression was identified as≥10 cells/HP. The enumeration data and measurement data were compared by t test andχ2 test, respectively. Overall survival （OS） and progression free survival （PFS） were estimated with the Kaplan-Meier method. Survival rates were compared by the Log-rank test. Statistical significance was determined at a level of P〈0.05. Results The median count of IL-6 positive cell number was 21 cells/HPF （range, 0-150 cells/HP）, and 61.29%（57/93） patients were positive. Positive IL-6 expression was mostly associated with fever and high Korean prognostic index （KPI） score （both P〈 0.05）. The serum c-reactive protein （CRP） level was （29.28 2.62） mg/L in IL-6 positive patients and （11.142.77） mg/L in IL-6 negative patients （t= -2.276, P= 0.025）. Patients with negative IL-6 expression had better survival, with 52.7 % of 5-year PFS rate,and 60.0 % of 5-year OS rate; the 5-year PFS and OS rates in those with IL-6 positive were 23.6 % and 27.1 %, respectively （both P= 0.001）. Conclusion The high expression of IL-6 in patients with NKTCL might be associated with adverse clinical feature and poor survival.

关 键 词：淋巴瘤 NK-T细胞 肿瘤微环境 白细胞介素6 INTERLEUKIN 6 

分 类 号：R733.1[医药卫生—肿瘤]