卵巢癌中Tiam1蛋白过表达的临床病理学意义  

Clinical implication of Tiam1 protein overexpression in ovarian carcinoma

作　　者：杨洋[1] 孙洁[1] 朱光 朴俊杰[1] 林贞花[1] 

机构地区：[1]延边大学医学院病理学教研室,延吉133002

出　　处：《临床与实验病理学杂志》2017年第9期945-949,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(61371067)

摘　　要：目的探讨卵巢癌中Tiam1过表达的临床病理学意义。方法采用免疫组化SP两步法检测Tiam1蛋白在98例浆液性卵巢癌组织、64例黏液性囊腺癌组织以及27例良性病变组织中的表达,并分析其过表达与卵巢癌临床病理特征的关系。应用Kaplan-Meier进行生存分析。结果 Tiam1蛋白主要表达于卵巢癌细胞胞质和胞核中。在浆液性卵巢癌组织中,Tiam1蛋白的阳性率和强阳性率分别为84.7%(83/98)和70.4%(69/98);在黏液性囊腺癌组织中,Tiam1蛋白的阳性率和强阳性率分别为87.5%(56/64)和73.4%(47/64),显著高于良性卵巢肿瘤组织(22.2%和7.4%)(P均<0.01)。Tiam1蛋白表达水平与卵巢癌组织学分级、FIGO分期和转移情况密切相关(P均<0.05);与患者年龄及绝经情况无关(P均>0.05)。Kaplan-Meier生存分析显示,Tiam1蛋白高表达的卵巢癌患者总生存期和无瘤生存期均明显低于Tiam1蛋白低表达患者(P均<0.05),且Tiam1蛋白高表达的转移性卵巢癌患者总生存期也明显低于Tiam1蛋白低表达患者(P<0.05)。结论 Tiam1蛋白在卵巢癌组织中明显高表达,其表达水平与卵巢癌患者的预后密切相关。Purpose To investigate the clinicopathological significance of Tiaml overexpression in ovarian carcinoma. Methods Tiaml protein was detected in 98 serous carcinomas, 64 ovarian mucinous cystadenocarcinomas and 27 benign ovarian tumors using immunohistochemical of SP two-step staining. Cor- relations between Tiaml overexpression and clinicopathological features of ovarian carcinoma were evaluated. Overall survival and disease-free survival rates of ovarian carcinoma patients were calculated by Kaplan-Meier method. Results Tiaml protein showed a cytoplasmic and nuclear staining pattern in ovarian car- cinoma. The positive rate and strongly positive rate of Tiaml protein were 84.7% （ 83/98 ） and 70.4% （ 69/98 ） in serous carcinoma, the positive rate and strongly positive rate of Tiaml protein were 87.5% （56/64） and 73.4% （47/64） in ovarian mucinous cystadenocarcinoma, both of which were significantly higher than those in benign ovarian tumor （ both P 〈 0. 01 ）. High-level expression of Tiaml was positively correlated with the histological grade, FIGO stage, and metastasis in ovarian carci- noma （ both P 〈 O. 05 ） , while independent of age and menopa- usal status （ both P 〉 0. 05 ）. Kaplan-Meier survival analysis showed that overall survival and disease-free survival of ovarian cancer patients with high expression of Tiaml protein were sig- nificantly lower than those of patients with lower expression of Tiaml protein （P 〈 O. 05 ）, overall survival in metastatic ovarian cancer patients with high expression of Tiaml protein was signifi- cantly lower than those in patients with low Tiaml protein ex- pression （ P 〈 0. 05 ）. Conclusion Tiaml expression is significantly high in ovarian carcinoma. Tiaml protein is closely associated with ovarian carcinoma progression, which may be a new potential prognostic biomarker and a therapeutic target for ovarian carcinoma.

关 键 词：卵巢肿瘤 TIAM1 免疫组织化学 预后 生存分析 

分 类 号：R737.31[医药卫生—肿瘤]