肝细胞癌中TLR3介导的间质微环境细胞改变  

Correlation of TLR3 with the alteration of stromal cells in tumor microenvironment of hepatocellular carcinoma

作　　者：张崇辉[1,2] 陈莉 

机构地区：[1]常州市儿童医院病理科,常州2130032 [2]南通大学医学院病理解剖学系,南通226001

出　　处：《临床与实验病理学杂志》2017年第9期987-991,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨肝细胞癌(hepatocellular carcinoma,HCC)中Toll样受体3(Toll-like receptor 3,TLR3)的表达及其与临床病理特征、间质微环境的相关性。方法采用专利技术(CN101319971B)制备人体HCC组织芯片,应用免疫组化SP法染色检测HCC细胞中TLR3的表达,同时检测间质中各项指标。采用多因素相关分析HCC中TLR3表达与其临床病理特征及间质微环境的关系。结果 HCC中TLR3的阳性率为71.57%,与癌脉管侵犯(P=0.001)、肝硬化背景(P=0.007)、Edmondson分级(P=0.001)、TNM分期(P=0.000)呈负相关;与癌乙型肝炎表面抗原(hepatitis B surface antigen,HBs Ag)阳性呈正相关(P=0.000);与间质浸润的T细胞(P=0.002)和自然杀伤(natural killer,NK)细胞(P=0.000)呈正相关;与癌相关肌纤维母细胞(carcinoma-associated fibroblasts,CAFs)(P=0.000)和微血管密度(microvessel density,MVD)(P=0.000)呈负相关。结论TLR3对HCC间质微环境具有十分重要的影响。Purpose To analyse the correlation between the Toll-like receptor 3 （ TLR3 ） expression and clinic pathologic factors, stromal microenvironment in hepatocellular carcinoma （HCC）. Methods The tissue microarrays of human HCC were prepared with self-owned patent technology. The expression of TLR3 in HCC cells as well as various indexes in HCC stroma was examined with immunohistochemistry of SP. The correlation between TLR3 expression with the clinic pathologic factors of HCC, and the correlation between TLR3 expression with the re- action of stromal ceils in HCC microenvironment were analyzed by multi-factor correlation analysis. Results The positive expression rate of TLR3 in HCC was 71.57%. The expression of TLR3 in HCC had negative correlation with vascular invasion （P=0. 001 ）, cirrhosis （ P = 0. 007 ）, Edmondson＇ s grades （ P = 0. 001 ） and staging of TNM （P =0. 000）. It had positive corre- lation with hepatitis B surface antigen （HBsAg） （P = 0. 000）. It had positive correlation with T cells （P = 0. 002） and natural killer （NK） cells （ P = 0. 000）. It had negative correlation with carcinoma-associated fibroblasts （CAFs） （ P = 0. 000） and mi- crovessel density （MVD） （ P = 0. 000）. Conclusion TLR3 has an important influence on the interstitial microenvironment of HCC.

关 键 词：肝肿瘤 肝细胞癌 TLR3 临床病理特征 微环境 相关性 

分 类 号：R735.7[医药卫生—肿瘤]