天然椰子油提取物对H_2O_2诱导的HSF细胞氧化损伤的保护作用  

Protective effects of virgin coconut oil extracts on oxidative damage of human skin fibroblasts cells induced by H_2O_2

作　　者：宋菲[1] 王挥[1] 唐敏敏[1] 沈晓君[1] 张玉锋[1] 邓福明[1] 

机构地区：[1]中国热带农业科学院椰子研究所海南省椰子深加工工程技术研究中心,海南文昌571339

出　　处：《日用化学工业》2017年第9期517-521,共5页China Surfactant Detergent & Cosmetics

基　　金：海南省自然科学基金资助项目(20163143);海南省重点研发计划资助项目(ZDYF2017129);中国热带农业科学院基本科研业务费专项资金资助项目(1630152017011)

摘　　要：采用倒置生物显微镜观察人皮肤成纤维细胞(HSF)的形态,通过MTT法检测细胞存活率,并通过检测细胞内活性氧(ROS)水平、总抗氧化能力和抗氧化酶活性探讨天然椰子油提取物对H_2O_2诱导的HSF细胞氧化损伤的保护作用。结果表明,天然椰子油提取物在质量浓度为12.5~50 mg/L范围内对H_2O_2诱导的HSF细胞氧化损伤具有保护作用,且呈浓度依赖性。天然椰子油提取物可以抑制H_2O_2引起的细胞形态损伤,提高细胞存活率,还可以通过降低细胞内ROS水平、提高细胞总抗氧化能力以及提高细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性,进而起到保护HSF细胞氧化应激损伤的作用。The human skin fibroblasts （HSF） cell morphology was observed by inverted biological microscope, the cell viability was detected by MTT method, and the intracellular ROS level, the intracellular total antioxidation capacity and the activity of antioxidation enzyme （SOD, CAT and GSH -Px） were examined for investigating the protective effects of virgin coconut oil extracts on oxidative damage of HSF cells induced by H202. Results show that the virgin coconut oil extracts could protect HSF cells from oxidative damage induced by n202 at mass concentration range of 12. 5 to 50 mg/L with a concentration dependent manner. Virgin coconut oil extracts could inhibit the cell morphological damage induced by H2O2 and improve the survival rate of the cells. In addition, virgin coconut oil extracts could protect HSF cells from oxidative damage induced by oxidative stress through decreasing the intracellular ROS level, increasing intracellular total antioxidant capacity and the enzyme activity of SOD, CAT and GSH- Px.

关 键 词：化妆品原料 天然椰子油 HSF细胞 抗氧化 抗衰老 

分 类 号：TQ658[化学工程—精细化工]