幽门螺杆菌细胞毒素促进胃黏膜分泌白介素8作用  

Cytotoxin of Helicobacter pylori pro-motes IL-8 secretion of gastric mu-cosa in vitro

作　　者：徐克强[1] 张万岱[2] 王继德[2] 李子旭 周殿元[2] 张亚历[2] 黄文峰 姜泊[2] 孙勇[2] 

机构地区：[1]中国人民解放军第157医院消化科,广东省广州市510510 [2]广东省广州市中国人民解放军第一军医大学南方医院全军消化内科研究所,510515

出　　处：《世界华人消化杂志》2002年第8期907-911,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨细胞因子在幽门螺杆菌(H.pylori)免疫病理机制中的作用.方法:48例患者(慢性浅表性胃炎37例,十二指肠溃疡(DU)11例,其中男性31例,年龄21-63岁)胃镜诊断明确后取胃窦及胃体活检,用于H.pylori诊断及体外组织培养.H.pylori诊断采用快速尿素酶试验、H.pylori培养及Warthin-Starry银染色,3项检查中任2项结果阳性诊断为H.pylori阳性.3块胃窦活检组织各加1mLRPMI1640,50mL·L-1CO2和37℃培养24h,ELISA法测上清中白介素(IL)-6,IL-8及肿瘤坏死因子(TNF)-α;Lowry改良法测活检组织蛋白含量.另8例RPMI1640中加入H.pylori细胞空泡毒素(VacA).结果以每克蛋白中ng(ng·g-1)或mg(μg·g-1)表示.结果:H.pylori阳性率69%.H.pylori阳性者胃黏膜培养上清中IL-6,IL-8和TNF-α含量均显著高于H.pylori阴性者,其中9例DU患者的IL-8含量为53(18-96)μg·g-1,显著高于慢性胃炎者的36(7-84)μg·g-1(P<0.01).培养液加入VacA后,IL-8的分泌量明显增高(50±38μg·g-1vs68±30μg·g-1,P<0.01).TNF-α的含量虽升高,但无显著差别,对IL-6的含量无影响.IL-8的含量与炎症程度及活动性显著相关(r=0.98,P<0.0025),IL-6和TNF-α的含量与炎症程度无明显关系(r=-0.26和-0.28,P>0.25),但活动性炎时高于非活动性炎者.结论:结果提示IL-8与炎细胞浸润密切相关,H.pylori细胞毒素对黏膜细胞因子的分泌有促进作用.AIM:To investigate effect of cytokines in theimmunopathogenesis of Helicobacter pylori (H. pylori).METHODS:Forty-eight patients (37 with chronic gastritisand 11 with duodenal ulcer (DU), and aged 21-63 yrs) werebiopsied under endoscopy for gastric mucosa culture in vitroand H. pylori detection. Rapid urease test, H. pylori cultureand Warthin-Starry silver stains were used for H. pyloridiagnosis. H. pylori was defined as positive when any 2 ofthese tests were positive. Each of 3 antrum pylori biopsieswas cultured in vitro for 24 h by adding 1mL RPMI 1640(37℃, 50mL·L-1 CO2). Supernatants were centrifuged andused for the detection of interleukin (IL) -6, IL-8 and tumornecrosis factor-a (TNF-α) by ELISA. Total proteins of thebiopsies were assayed by a modified Lowry method. An-other 8 cases were cultured with RPMI 1640 added by H.pylori vacuolating cytotoxin (VacA). The results were ex-pressed as ng orμg per gram protein (ng·g-1 or μg·g-1).RESULTS: Sixty-nine percent of the patients were H. py-lori positive. IL-6, IL-8 and TNF-α contents in the superna-tants of cultured mucosa were all significantly higher in H.pylori positive patients than those in H. pylori negativepatients. Among them, IL-8 content of 9 cases of DU pa-tients was 53 (18-96) μg·g-1, which was significantly higherthan 36(7-84) μg·g-1 of chronic gastritis patients (P <0.01).Addition of VacA into culture media could significantly pro-mote IL-8 secretion from gastric mucosa (50±38 μg·g-1 vs68±30 μg·g-1, P <0.01). TNF-α content was not significantlyincreased, and IL-6 was not changed. IL-8 was closely asso-ciated with the inflammation grading and activity (r =0.98, P<0.0025). No correlation was observed between IL-6, TNF-α and inflammation grading (r =-0.26 and-0.28, P >0.25),but they were higher in active gastritis than those in non-active gastritis.CONCLUSIONS:IL-8 is closely associated with the infil-tration of inflammatory cells, and H. pylori cytotoxins areresponsible for the increased secretion of cytokines.

关 键 词：幽门螺杆菌细胞毒素 胃黏膜 白介素8 免疫学 炎细胞浸润 

分 类 号：R573[医药卫生—消化系统]