核桃举肢蛾对植物挥发物的触角电位反应  

Electroantennogram Responses of Atrijuglans hetaohei to Plant Volatiles

作　　者：李天凤 郭安柱 张婷[1] 唐光辉[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]洛南县核桃研究所,陕西洛南726100

出　　处：《西北林学院学报》2017年第5期161-167,共7页Journal of Northwest Forestry University

基　　金：国家自然科学基金项目(31270690);陕西省攻关项目(2017NY-105);校推广项目(TGZX2017-22)

摘　　要：探讨核桃举肢蛾(Atrijuglans hetaohei)对植物挥发物的触角电位(electroantennogram,EAG)反应,为研发核桃举肢蛾植物源引诱剂提供依据。采用电生理学方法测试了核桃举肢蛾对38种植物挥发物的EAG反应。结果表明,测试的38种化合物中,核桃举肢蛾对脂类化合物反应较强,其次为芳香类化合物,对萜烯类化合物反应相对较弱。雌虫对多数供试化合物的EAG反应强度大于雄虫,雌虫对乙酸己酯和反-2-己烯乙酸酯的EAG反应值最高,雄虫对反-2-己烯醇、反-2-己烯醛的EAG反应值最高且大于雌虫。在芳香类化合物中,苯甲醛可引起雌雄成虫明显的EGA反应。在萜类化合物中,雌虫对香叶醇和芳樟醇的EAG反应较明显,雄虫对柠檬醛EAG反应值相对较高。剂量反应曲线表明,在测定的浓度范围内,核桃举肢蛾雌雄成虫对反-2-己烯醇、反-2-己烯醛、顺-3-己烯醇、顺-3-己烯乙酸酯、乙酸己酯、己酸乙酯和苯甲醛的EAG反应变化趋势相同,对反-2-己烯乙酸酯、香叶醇和芳樟醇反应变化趋势存在一定差异。核桃举肢蛾雌雄成虫对不同植物挥发物的EAG反应存在差异,雌虫对多数植物挥发物更敏感具有重要生态适应意义,利于其选择寄主、产卵场所等行为。研究结果为植物挥发物在核桃举肢蛾防治中的利用提供了线索。The moth Atrijuglans hetaohei is a major pest to walnut fruit. It is useful to screen out the effective compounds of plant volatiles for developing an alternative method for monitoring and controlling A. hetaohei. By using electroantennogram （EAG） instrument, this study examined the EAG responses of A. hetaohei adults to 38 kinds of plant volatiles. The results indicated that A. hetaohei showed stronger EAG responses to aliphatics, followed by aromatics and monoterpene hydrocarbons. Females were more sensitive to most plant volatiles, such as hexyl acetate and （E）-2-hexenyl acetate. Meanwhile,males were more sensitive to （E）-2-hexenol, （E）-2-hexenal. in aromatic compounds, the relative EAG values of 245.86% and 190.77% revealed that significant EGA reactions of male and female could be induced by benzene formalde- hyde. Among monoterpene hydrocarbons,while females were more sensitive to geraniol and linalyl acetate, males were more sensitive to citral. Dosage response curves of EAG of A. hetaohei showed that the trends of reactions of females and males were the same to （E）-2-hexenol, （E）-2-hexenal, （Z）-3-hexenyl acetate, （Z）-3-hexenol,hexyl acetate,ethyl caproate and benzene formaldehyde. However, the trends of their reac- tions to （E）-2-hexenyl acetate, geraniol and linalyl acetate showed differences. Sensitivity differences between both sexes to plant volatiles suggested that ecological adaptation of this species and the females were more sensitive to most plant volatiles,which would be useful for their finding of hosts. The results would provide a reference for developing attractive chemicals for A. hetaohei in the fields.

关 键 词：核桃举肢蛾 植物挥发物 触角电位 剂量反应 

分 类 号：S763.3[农业科学—森林保护学]