青海汉、回族胎膜早破与MMP-3 rs35068180、-8 rs11225395基因多态性（英文）  

The correlation study between MMP-3 rs35068180 and-8 rs11225395 gene polymorphism and premature rupture of membranes in Qinghai Han- and Hui-Chinese pregnant woman

作　　者：白玉芳[1] 路丽 祁存秀 刘文翠 沈国平[3] 巩海凤 朱雪玲 王荣华[4] BAI Yu-fang LU Li QI Cun-xiu Liu Wen-cui SHEN Guo-ping GONG Hai-feng ZHU Xue-ling WANG Rong-hua(Dept of Obstetrics, Qinghai University Affiliated Hospital Qinghai University Graduate School Qinghai University Medical College Central Sterile Supply Department of Qinghai University Affiliated Hospital, Qinghai ,810001)

机构地区：[1]青海大学附属医院产科 [2]青海大学研究生院 [3]青海大学医学院 [4]青海大学附属医院消毒供应中心,青海810001

出　　处：《中国高原医学与生物学杂志》2017年第3期157-163,共7页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省应用基础研究项目(2016-ZJ-762);青海省卫计委项目(2015)

摘　　要：目的利用PCR-RFLP及测序法分析青海地区回、汉族胎膜早破孕妇MMP-3rs35068180、-8 rs11225395基因多态性特点及是否具有民族差异性。方法提取胎膜早破孕妇和对照组组织DNA,利用PCR-RFLP及琼脂糖凝胶电泳、测序法检测基因多态性,并行相关性分析。结果 MMP-3 rs35068180A/A、A/Del、Del/Del基因型频率在汉族对照组和胎膜早破组中分别是80.53%、17.70%、1.77%和100.00%、0.00%、0.00%,两组基因型频率分布具有显著差异性(P=0.000);在回族中分别是81.61%、16.09%、2.30%和100.00%、0.00%、0.00%,两组基因型频率呈现显著差异性(P=0.003)。MMP-8 rs11225395三种基因型A/A、A/G、G/G在汉族对照组和胎膜早破组中基因型频率分别为17.14%、31.43%、51.43%和14.16%、57.52%、28.32%,两组相比,基因型频率分布具有显著性差异(P=0.002);回族对照组和胎膜早破基因型频率分布分别为17.24%、32.76%、50.00%和14.94%、57.47%、27.59%,两组基因型频率相比具有显著性差异(P=0.009)。结论 MMP-3 rs35068180、-8 rs11225395基因多态性在青海汉族、回族胎膜早破者间无民族差异性。同时,青海汉族、回族对照组和胎膜早破组中MMP-3 rs35068180、-8 rs11225395基因频率分布差异无统计学意义。Objective To analyze the polymorphism of matrix metalloprotease-3 （MMP-3）rs35068180 and-8rs11225395 gene in pregnant women with premature rupture of membranes in Han- and Hui-Chinese in Qinghai province by PCR-RFLP and sequencing.Methods Genome DNA was extracted from preterm premature rupture of membranes and control group.The polymorphism of the gene was analyzed by PCR-RFLP, agarose gel electrophore- sis and sequencing.Resets The frequency of MMP-3 rs35068180A/A, A/Del, Del/Del genotype was 80.53%, 17.70%, 1.77% and 100.00% ,0.00% ,0.00% ,respectively,in the Han control and the premature rupture of mem- branes groups, the genotype frequency was significantly different between two groups（ P = 0.000）. Meanwhile the fre- quency of genotype was 81.61%, 16.09% ,2.30% and 100.00% ,0.00% ,0.00% ,respectively,in the control and the premature rupture of membranes group in Hui Chinese, and the genotype frequency was obviously different between two groups（P=0.003）.The frequency of MMP-8 rs11225395 A/A,A/G, G/G genotype was 17.14%,31.43%, 51.43% and 14.16% ,57.52%,28.32%, respectively, in the Han control and the premature rupture of membranes groups, the genotype frequency was significantly different between two groups （P = 0.002）.Meanwhile the frequency of genotype was 17.24% ,32.76% ,50.00% and 14.94% ,57.47% ,27.59% ,respectively,in the control and the pre- mature rupture of membranes group in Hui Chinese, and the genotype frequency was obviously different between two groups （ P = 0.009）. Conehmiort The polymorphisms of MMP- 3 rs35068180 and - 8 rs 11225395 may be related to the premature rupture of membranes in Qinghai Han and Hui Chinese pregnant women. Meanwhile, the allele fre- quencies of MMP-3 rs35068180 and -8 rs11225395 were not specific between Han and Hui nationality.

关 键 词：MMP 基因 基因多态性 胎膜早破 青海 回族 汉族 

分 类 号：R394[医药卫生—医学遗传学]