PM2．5对大鼠COPD模型肺泡灌洗液IL-8、IL-17、TNF-α影响及意义  

Effects and significance of IL-8,1L-17 and TNF-α in bronchoalveolar lavage fluid of COPD rats with PM2.5

作　　者：吴亮亮 陈乐蓉[1] 袁小兰[1] 彭建萍[1] 

机构地区：[1]江西省胸科医院呼吸科,南昌330006

出　　处：《国际呼吸杂志》2017年第17期1300-1304,共5页International Journal of Respiration

摘　　要：目的初步研究BALF IL-8、IL-17、肿瘤坏死因子α（tumor necrosis factor-α，TNPd）在PM2．5干预下COPD大鼠（chronic obstruction pulmonary disease）模型中水平变化，研究其在COPD发病中的作用。方法48只雄性Wistar大鼠随机分成2个组，即正常组（A组），COPD组（B组），每组24只，采用气管滴注的染毒方式，对A组、B组大鼠气管滴注无菌生理盐水及PM2．5染毒，每天染毒1次，连续3d，在最后一次染毒24h后处死动物，分析BALF中IL8、IL-17、TNF-α的含量。结果①与气管滴人生理盐水的A组相比，气管滴人PM2．5的B组BALF中IL-8浓度显著升高，差异均有统计学意义（P〈O．05）；②随着大鼠染毒剂量增加，BALF中IL-8水平亦有所增加，呈正相关；③气管滴入PM2．5的B组BALF中IL17、TNF-α水平亦有升高，高于气管滴人生理盐水A组大鼠IL17、TNF-α水平，两组比较差异均有统计学意义（P〈0．05）。结论IL-8、IL-17、TNF-α可能参与了COPD气道及系统炎症，但IL8可能是参与COPD急性加重期发生、发展的重要炎症因子，并且在PM2．5干预下IL8、IL-17、TNF-α相互作用贯穿与COPD的发生、发展，对评估COPD严重程度及预后具有重要的临床意义。Objective To study the levels of BALF interleukin 8 （IL-8）, IL-17 and tumor necrosis factor-α （TNF-α） in the chronic obstruction pulmonary disease （COPD）, and role of COPD in the pathogenesis. Methods 48 male Wistar rats were randomly divided into 2 groups：A group and B group, 24 rats in each group by tracheal infusion of the way, A, B two groups of rats by intratracheal instillation of sterile saline and PM2.5 were exposed 1 times every day, for 3 consecutive days, the dead animal in the last 24 hours after exposure, analysis of bronchoalveolar lavage fluid in IL--8, IL-17, TNF-a content of alpha. Results ①Compared with A group of tracheal drip saline, the concentration of IL 8 in BALF of bronchoalveolar lavage fluid in B group with PM2.5 was significantly increased, and the difference was statistically significant （ P d0.05）. ②With the increase of rat exposure dose, the IL 8 level in BALF also increased, which was positively correlated; ③IL-17 and TNF-α levels in BALF of bronchoalveolar lavage fluid in COPD group were also increased, higher than IIJ-17, TNF-α level of tracheal drip into normal saline group, the difference between the two groups were statistically significant （P《0.05 ）. Conclusions IL-8,IL-17,TNF-α may be involved in COPD airway and systemic inflammation, But IL-8 may be involved in COPD acute exacerbation of the occurrence and development of important inflammatory factors, IL-8, IL 17 and TNF-α interact with the occurrence and development of COPD in PM2.5 intervention, it is of great clinical significance to evaluate the severity and prognosis of COPD.

关 键 词：大气细颗粒物PM2.5 COPD模型 白介素8 白介素17 肿瘤坏死因子α 

分 类 号：R-332[医药卫生] R563.9