对节白蜡树叶乙酸乙酯提取物的GC-MS分析及其结构初探  

GC-MS analysis and preliminary study on structures of extracts from Fraxinus hupehensis leaves

作　　者：张良菊 徐志红[1,2] 徐慧慧[1,2] 李俊凯[1,2] 吴清来[1,2] 

机构地区：[1]长江大学农学院,湖北荆州434025 [2]长江大学农药研究所,湖北荆州434025

出　　处：《经济林研究》2017年第3期186-192,228,共8页Non-wood Forest Research

基　　金：国家自然科学基金项目(30971948);湖北省自然科学基金项目(2014CF105)

摘　　要：为了研究对节白蜡的抗病原因,以探寻新的生物农药,在前期有关对节白蜡树叶提取物杀菌活性研究的基础上,对其乙酸乙酯提取物及柱层析分离组分进行了GC-MS分析,初步确认了各组分所含化合物的可能结构。结果表明:乙酸乙酯提取物中面积归一含量超过1%的化合物组分有18个,其中3号化合物的面积归一含量为17.13%,其结构为4-羟苯基乙醇;经过柱层析分离后各组分所含化合物要相对集中和更加纯化些,其中组分A所含物质主要有两个,化合物4-羟苯基乙醇的含量达到了81.3%,这是相对比较纯的物质;作者据其已有的相关研究结果(组分A整体表现出的杀菌活性要优于其他组分)分析认为,4-羟苯基乙醇作为对节白蜡树叶中所含的重要化合物,在对节白蜡的抗病方面起到了重要作用。In order to study the anti-disease cause of Fraxinus hupehensis in search of a new bio-pesticide, on the basis of the early bactericidal activity researches on extract from F. hupehensis leaves, ethyl acetate extracts and its column chromatography isolation ingredients were analyzed by GC-MS, and the possible structures of compounds contained in each ingredient were preliminarily identified. The results showed that, in ethyl acetate extracts, area normalization contents of 18 compound ingredients were over 1%, among which the No.3 compound ingredient had area normalization contents of 17.13% and had the structure of 4-hydroxyl phenyl ethanol. Through column chromatography isolation, compounds contained in each ingredient were more concentrated and purer. Ingredient A contained two substances, and its 4-hydroxyl phenyl ethanol content reached 81.3%, which was relatively pure. According to available relevant research results, the bactericidal activity shown by Ingredient A was better than that of the others. So 4-hydroxyl phenyl ethanol, as a important compound in F. hupehensis leaves, played an important role in the anti-disease ability ofF. hupehensis.

关 键 词：对节白蜡 提取物 杀菌活性 气质联用 结构 

分 类 号：S608[农业科学—园艺学] S792.41