花粉与P123双模板制备的新型多级孔生物活性玻璃对MG63细胞增殖和分化的影响  

Effect of New Type Multistage Aperture Bioactive Glasses Prepared by Pollen and P123 Dual Template on Proliferation and Differentiation of MG63 Cells

作　　者：汪洋[1] 申玉芹[2] 凌新连 刘宇妍 秦甜甜 刁梦雪 孙新华[1] WANG Yang SHEN Yu-qin LING Xin-lian LIU Yu-yan QIN Tian-tian DIAO Meng-xue SUN Xin-hua(Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China Department of Periodontology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China College of Food Science and Engineering, Jilin University, Changchun 130000, China.)

机构地区：[1]吉林大学口腔医学院正畸科吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021 [2]吉林大学口腔医学院牙周病科,吉林长春130021 [3]吉林大学食品科学与工程学院,吉林长春130000

出　　处：《口腔医学研究》2017年第9期920-923,共4页Journal of Oral Science Research

基　　金：国家自然科学基金面上项目(编号:81371153);吉林省科技厅自然科学基金项目(编号:20150101173JC);白求恩前沿交叉学科创新项目(编号:20131080031)

摘　　要：目的:检测花粉与P123双模板制备的新型多级孔生物活性玻璃对人前成骨细胞系MG63增殖及成骨分化的影响。方法:在材料工作浓度为0.2g/mL的浸提液(含10%胎牛血清的DMEM)中,培养MG63细胞至1、3、5d,分别采用MTT比色法检测细胞增殖水平;碱性磷酸酶试剂盒检测细胞内ALP活性;实时定量PCR法测定成骨标志性基因RUNX-2、SP7、COL-1的mRNA表达水平。结果:花粉与P123双模板制备的新型多级孔生物活性玻璃可促进MG63细胞增殖,增强ALP活性表达,上调MG63标志性基因RUNX-2、SP7、COL-1的mRNA表达水平。结论:以花粉与P123双模板制备的新型多级孔生物活性玻璃具有细胞毒性低,且具有促进成骨细胞成骨向分化的潜能。Objective： To investigate the effects of new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template on the proliferation and differentiation of MG63 cells. Methods： MG63 cells were cultured in the material leach liquor （0.2g/ml working concentration, DMEM with 10% fetal bovine serum） for 1, 3, and 5 days. MTT colorimetric method was used to detect the level of cell proliferation. Alkaline phosphatase kits was used to measure the ALP activity. Real-time PCR was used to analyze the mRNA expression of osteogenesis marker, i.e. RUNX-2, SP7, and COL-1. Results： The material promoted the proliferation of MG63 cells, enhanced the activity of ALP, up-regulated the mRNA expression level of markers gene RUNX-2, SP7, and COL-1. Conclusion： The new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template has low cytotoxicity and the potential to promote the osteogenetic differentiation.

关 键 词：新型多级孔生物活性玻璃 MG63细胞 增殖 分化 

分 类 号：R783.1[医药卫生—口腔医学]