腺病毒介导HGF转染脂肪干细胞复合温敏型可注射水凝胶对兔颞下颌关节骨关节病髁突软骨的修复作用  

Therapeutic Effect of Adenovirus-mediated HGF Transfection of ADSCs Combined with Injectable Hydrogel on the Repair of Temporomandibular Joint Arthritic Condylar Cartilage in Rabbits

作　　者：张广德[1] 李荣亮[1] 岳从雷 杨世茂[2] 郭延伟[1] 房殿吉 ZHANG Guang- de LI Rong- liang YUE Cong-lei YANG Shi--maoz GUO Yan-wei FANG Dian-ji(Department of Oral and Maxil- lofacial Surgery, Jining Hospital of Stomatology , Jining 272000, China Department of Oral and Maxillofacial Surgery, Jinan Hospital of Stomatology , Jinan 250000, China Department of Oral Surgery, Institute of Dental Surgeons, Shanghai Minhang , Shanghai 201100, China.)

机构地区：[1]济宁口腔医院口腔颌面外科,山东济宁272000 [2]济南市口腔医院口腔颌面外科,山东济南250000 [3]上海闵行区牙病防治所口腔颌面外科,上海201100

出　　处：《口腔医学研究》2017年第9期924-927,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81170935)

摘　　要：目的:探讨肝细胞生长因子(HGF)转染脂肪干细胞复合水凝胶支架材料对促进兔颞下颌关节炎病理状态微环境中受损软骨修复的意义。方法:培养兔脂肪干细胞,利用腺病毒介导对其进行HGF转染并制备ADSCs/HGF/水凝胶支架材料。兔颞下颌关节上腔为注射区并分为5组,A组为空白对照组,B组为模型组,C组为单纯细胞治疗组,单纯脂肪干细胞腔内注射,D组为干细胞复合材料组,为造模后腔内注射干细胞复合可注射水凝胶支架材料,E组为转染后干细胞复合水凝胶注射。所有材料移植成功后各组动物分别于用药第3、9周后取材,制备标本并行髁突软骨扫描电镜、组织学观察及实时定量PCR检测,采用SPSS17.0软件包对数据进行方差分析及t检验。结果:扫描电镜与组织学观察可见,D、E组软骨病变区改善情况优于B、C组。与模型组比较,实时定量PCR结果显示,E组MMP-13表达与空白组相对持平但低于C、D组,差异有显著性(P<0.05);E组TIMP-1表达与空白组相对持平但高于C、D组,差异有显著性(P<0.05)。结论:腺病毒介导HGF转染脂肪干细胞复合可注射型水凝胶对兔颞下颌关节骨关节病受损软骨具有明显修复作用。Objective： To investigate the therapeutic effect of Ad-HGF-GFP of ADSCs combined with injectable hydrogel on the repair of damaged cartilage in the microenvironment of rabbit temporomandibular arthritis. Methods： ADSCs were isolated from rabbits and cultured in vitro. Cells were transfected with adenovirus-mediated HGF and injectable hydrogel were prepared. Then animals were randomly divided to five groups： group A （blank control）; group B; group C （treated with ADSCs）; group D （treated with ADSCs combined with injectable hydrogel）; group E （treated with Ad-HGF-GFP-ADSCs combined with injectable hydrogel）. All animals were sacrificed 3 or 9 weeks after surgery, and condylar cartilages were harvested and analyzed by scanning electron microscope, histomorphometric analysis, and real-time PCR. Statistical analysis was performed with SPSS 17.0 software and statistically significant differences were assessed by t-test. All statistical analysis was considered significant when P〈0.05. Results： Electron microscope scanning and histomorphometric analysis indicated improvement of condylar cartilage in group D and E, compared to those in the group B and C. Real-time PCR showed that the expression of mmp13 gene in group E and A was comparable but decreased in group C and D, the difference was statistically significant. In contrast, the expression of TIMP-1 gene in group E and A was comparable but increased in group C and D, and the difference was statistically significant. Conclusion： Adenovirus-mediated HGF transfection of ADSCs combined with injectable hydrogel could promote the repair of temporomandibular joint arthritic condylar cartilage in rabbits.

关 键 词：脂肪干细胞 腺病毒 肝细胞生长因子 温敏型可注射水凝胶 颞下颌关节 骨关节病 

分 类 号：R782.6[医药卫生—口腔医学]