喷砂酸蚀对钛铌锆锡合金表面成骨细胞粘附、增殖和分化的影响  

Effect of Sandblast and Acid Etching on Attachment,Proliferation,and Differentiation of Osteoblasts on Ti-24Nb-4Zr-7.9Sn

作　　者：冯帅儒 刘鑫 潘爽[1] 刘会梅[1] 牛玉梅[1] FENG Shuai-ru LIU Xin PAN Shuang LIU Hui-mei NIU Yu-Mei(Department of Endodontics, Stomatology College of Harbin Medical University, Harbin 150001, China.)

机构地区：[1]哈尔滨医科大学口腔医学院牙体牙髓病科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2017年第9期933-937,共5页Journal of Oral Science Research

基　　金：黑龙江省卫生厅科研课题资助项目(编号:2011-051)

摘　　要：目的:研究喷砂酸蚀(SLA)对钛及钛铌锆锡合金(Ti-24Nb-4Zr-7.9Sn,TNZS)表面形貌的影响,观察合金的形貌学特征,评价其生物相容性。方法:将试样分为钛机械打磨并抛光组(Ti组),钛铌锆锡机械打磨并抛光组(TNZS组),钛喷砂酸蚀组(Ti-SLA组)和钛铌锆锡喷砂酸蚀组(TNZS-SLA组),共4组。通过扫描电镜观察各组试样的表面形貌,3D激光共聚焦显微镜和接触角测量仪测量各组试样表面的粗糙度与亲水性。接种MC3T3-E1小鼠前成骨细胞于各组试样表面,检测细胞在试样表面的粘附、增殖与矿化的能力,评估其生物相容性。结果:SLA处理后在材料表面形成纳米级及微米级的凹坑,产生均匀分布的粗糙结构,经过处理后材料仍保持亲水性。细胞在TNZS组上短期粘附明显高于其它组(P<0.05),TNZS-SLA组细胞增殖、分化能力均明显高于其它组(P<0.05)。结论:喷砂酸蚀后材料表面相对于光滑材料表面能更有效的促进成骨细胞在其表面增殖、分化,经喷砂酸蚀的钛铌锆锡合金具有良好的细胞相容性。Objective： To evaluate the effect of sandblast and acid etching treatments on attachment, proliferation, and differentiation of osteoblasts on Ti and Ti-24Nb-4Zr-7.9Sn （TNZS）. Methods： Samples were divided into four groups： Ti group, Ti-SLA group, TNZS group, and TNZS-SLA group. Ti and TNZS groups were polished with different rough emery papers, Ti-SLA and TNZS-SLA groups were polished with different rough emery papers and sandblasted by Al2O3 particles and acid etched by mixture of hydrochloric acid and sulfuric acid. Surface morphology of the samples was analyzed by scanning electron microscope （SEM）. Contact angles were examined by Tensiometer and Contact Angle Meter. Roughness of samples was examined by 3D laser scanning confocal microscope. MC3T3-E1 were seeded on the surface of four groups of samples and evaluated by different means. Results： Ti-SLA and TNZS-SLA surfaces exhibited micro-topographical and nano-topographical modifications and roughness distributed evenly. After treatment, four groups had similar hydrophilicity. The adhesion of MC3T3-E1 preosteoblasts showed that Ti and TNZS had higher attachment than SLA groups （P〈0.05）. TNZS-SLA showed the highest proliferation and differentiation （P〈0.05）. Conclusion： SLA surface treatment generates porous microstructures and nanostructures. It can enhance proliferation and differentiation of osteoblasts. TNZS alloy treated by sandblast and acid etching possesses good cytocompatibility.

关 键 词：喷砂酸蚀 MC3T3-E1 T-24Nb-4Zr-7.9Sn 细胞增殖与分化 

分 类 号：R783.1[医药卫生—口腔医学]