铁调素和IL-6在非酒精性脂肪性肝病大鼠模型肝脏炎症进程中的表达水平及相关性分析  

Expression of hepcidin and interleukin-6 and their correlation in liver inflammation process in a rat model of nonalcoholic fatty liver disease

作　　者：赵睿[1] 李娟[1] 王亮[1] 孔银[1] 丁洁[1] 王娟霞[1] 李光明[1] 张岭漪[1] ZHAO Rui LI Juan WANG Liang et al(Department of Eepatology, Lanzhou University Second Hospital, b~nzhou 730030, Chin)

机构地区：[1]兰州大学第二医院肝病科,兰州730030

出　　处：《临床肝胆病杂志》2017年第9期1774-1777,共4页Journal of Clinical Hepatology

基　　金：2014年甘肃省自然科学基金项目(145RJZA147);中央高校基本科研业务费专项资金(223000-861240)

摘　　要：目的利用非酒精性脂肪性肝病(NAFLD)大鼠模型观察铁调素(Hepc)、IL-6在肝脏炎症进程中表达水平的变化。方法NAFLD肝组织标本均来自本课题组前期利用高脂乳剂灌胃法成功构建的大鼠NAFLD模型(M组)及同期生理盐水灌胃替代构建的正常大鼠对照组(C组)。Hepc、IL-6单克隆抗体应用免疫组织化学法分别检测4、8、12周M组(M_4、M_8、M_(12))和12周C组大鼠肝组织中Hepc、IL-6的表达水平,同期评估各组肝脂肪炎症程度积分。检测2组大鼠肝组织Hepc和IL-6的表达水平,并计算肝组织NAS积分。多组间比较采用单因素方差分析,组间进一步两两比较采用LSD-t相关法。相关性分析均采用Pearson相关法。结果随试验周期延长,Hepc和IL-6在M_4组、M_8组、M_(12)组表达水平呈逐渐升高的趋势,且与C_(12)组相比差异均有统计学意义(Hepc:0.372±0.216、1.213±0.193、2.390±0.192 vs 0.166±0.192;IL-6:0.499±0.218、1.290±0.210、2.644±0.441 vs0.240±0.109,P值均<0.05);M组大鼠肝组织Hepc和IL-6表达呈正相关(r=0.944,P<0.05);M组大鼠肝组织Hepc和IL-6表达水平均与NAS呈正相关(r值分别为0.927和0.907,P值均<0.001)。结论初步观察到实验性NAFLD炎症进程可影响肝组织Hepc、IL-6表达且Hepc与肝组织炎症损伤因子IL-6表达强度相关性明显。Hepc和IL-6作为NAFLD疾病进展的预测因子值得进一步研究。Objective To investigate the changes in the expression of hepcidin（ Hepc） and interleukin-6（ IL-6） in liver inflammation process in a rat model of nonalcoholic fatty liver disease（ NAFLD）. Methods The rats were given high-fat emulsion by gavage to establish the model of NAFLD（ group M） and normal rats were given normal saline by gavage as controls（ group C）. Liver tissue samples were collected from these rats,and immunohistochemistry was used to measure the expression of Hepc and IL-6 in liver tissue in group M at 4,8,and 12weeks（ M_4,M_8,and M_（12）groups） and in group C at 12 weeks（ C_（12）group）. The NAFLD activity score（ NAS） was assessed for both groups.The expression of Hepc and IL-6 in liver tissue was measured,and NAS was calculated. A one-way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for comparison between two groups. Pearson correlation analysis was also performed. Results Over the course of treatment,the M_4,M_8,and M_（12） groups had gradual increases in the expression of Hepc and IL-6,and there were significant differences between these groups and the C_（12）group（ Hepc： 0. 372 ± 0. 216/1. 213 ± 0. 193/2. 390± 0. 192 vs 0. 166 ± 0. 192,all P〈0. 05; IL-6： 0. 499 ± 0. 218/1. 290 ± 0. 210/2. 644 ± 0. 441 vs 0. 240 ± 0. 109,all P〈0. 05）. In group M,the expression of Hepc was positively correlated with that of IL-6（ r = 0. 944,P〈0. 05）,and the expression Hepc and IL-6 was positively correlated with NAS（ r = 0. 927 and 0. 907,both P〈0. 001）. Conclusion Based on the results of this preliminary study,liver inflammation process in NAFLD can upregulate the expression of Hepc and IL-6 in liver tissue,and Hepc is significantly correlated with the inflammatory factor IL-6. Further studies are needed to investigate the roles of Hepc and IL-6 in predicting the progression of NAFLD.

关 键 词：脂肪肝 铁调节蛋白质类 白细胞介素6 

分 类 号：R-332[医药卫生] R575.5