改良穿刺法建立大鼠无血预充非停跳体外循环后认知功能障碍模型  

Establishment of rats ' cardiopulmonary bypass POCD model by meliorated puncture method

作　　者：段铭杰 孙莹杰[1] 郑翔 李思沁 屈鹏霞 Duan Ming-jie Sun Ying-jie Zheng Xiang Li Si-qin Qu Peng-xia(Department of Anaesthesiology, General Hospital of Shenyang Military Region, Shenyang 110016, China)

机构地区：[1]沈阳军区总医院麻醉科,沈阳100016 [2]山东省千佛山医院,济南250014

出　　处：《中国体外循环杂志》2017年第3期179-184,共6页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然科学基金资助项目(81471121)

摘　　要：目的寻找建立大鼠无血预充非停跳体外循环术后认知功能障碍模型的简便方法。方法 SD大鼠20只,0.2%戊巴比妥钠溶液腹腔注射麻醉。经由锁骨上入路右上腔静脉穿刺引流,尾动脉穿刺灌注,隐动脉穿刺监测血压。术中持续监测心率、血氧饱和度及肛温。转流流量维持在100 ml/(min·kg),转流中根据血气结果适当补充葡萄糖酸钙、潘南金(门冬氨酸钾镁注射液)和碳酸氢钠调整血气指标,体外循环转流60 min。结果 16只大鼠成功存活,存活大鼠术后一天均可完成水迷宫训练。结论此种改良穿刺方法建立的体外循环模型,创伤小、简便易行,可用于术后行为学检测。Objective To explore a convenient method of cardiopulmonary bypass POCD model in rats.Methods Twenty Sprague Dawley rats were anesthetized with Pelltobarbitalum Natricum i.p.Right upper vena cava was punctured for the drainage of blood and caudal artery for perfusion.Heart rate,anal temperature and blood oxygen saturation were monitored.Meanwhile,the blood gas indexes were measured and adjusted during the operation.The flow of CPB was maintained 100 ml/（ min·kg） and time interval of CPB lasted for 60 mins.Results 16 models were established successfully and all of which were able to complete the water maze training the day after operation.Conclusion The CPB model established by the improved puncture method can shorten the operation time and reduce the difficulty of operation with more convenience and less trauma.

关 键 词：静脉穿刺 认知功能障碍 体外循环 水迷宫 

分 类 号：R-332[医药卫生] R654.1