关于子宫内膜异位症患者内膜中TLR4、MyD88、NF-κB表达的临床研究  

Study on the expression of TLR4,MyD88 and NF-κB in the endometrial of patients with endometriosis

作　　者：高原 杨文[1] 

机构地区：[1]江苏省连云港市第一人民医院,222000

出　　处：《检验医学与临床》2017年第18期2716-2717,共2页Laboratory Medicine and Clinic

摘　　要：目的通过观察子宫内膜异位症(EMs)患者内膜中TLR4、MyD88、NF-κB的表达,探究发生该疾病的可能机制。方法选取120例EMs患者,其中Ⅰ、Ⅱ期33例,Ⅲ、Ⅳ期87例;取异位内膜120例,在位内膜89例,取同期行宫腔镜手术患者正常内膜组织100例为正常对照组。采用蛋白免疫印迹法(Western blot)测定TLR4、MyD88、NF-κB的表达情况。结果异位内膜组、在位内膜组TLR4、MyD88、NF-κB蛋白表达水平均高于对照组,差异有统计学意义(P<0.05),异位内膜组高于在位内膜组(P<0.05)。不同分期EMs患者内膜组织中TLR4、MyD88、NF-κB表达水平比较,差异无统计学意义(P>0.05)。结论TLR4、MyD88、NF-κB通路可能是EMs发病的重要机制。Objective To study the expression of TLR4,MyD88 and NF-κB in the endometrium of patients with endometriosis（Endometriosis,EMs）,and explore the possible mechanism of the disease.Methods 120 cases of patients with endometriosis,including stageⅠandⅡwere 33,stageⅢ and Ⅳ were 87,120 cases of ectopic endometrium,eutopic endometrium of 89 cases from the same patients underwent hysteroscopic surgery in 100 cases of normal endometrium as normal control group.The expression of TLR4,MyD88 and NF-κB were determined by Western blot（Western blot）.Results The expression levels of TLR4,MyD88 and NF-κB in ectopic endometrium group were higher than those in control group,the difference were statistically significant（P0.05）,and ectopic endometrium group were significantly higher than those in the control group（P0.05）.There were no significant difference in the expression of TLR4,MyD88 and NF-κB in endometrial tissues of patients with different stages of EMs（P0.05）.Conclusion TLR4,MyD88,NF-κB pathway may be an important mechanism for the pathogenesis of endometriosis.

关 键 词：子宫内膜异位症 TLR4 MYD88 NF-ΚB 

分 类 号：R730.5[医药卫生—肿瘤]