3种金属全冠修复对犬牙龈细胞Bcl-2和Bax表达的影响  

Influence on Bcl-2 and Bax in Gingival Cells of the Dogs After Restored With Three Types of Full Metal Crown

作　　者：李骏[1] 陈林[1] 范芹[1] 龚晓娇 杨成雪 LI Jun CHEN Lin FAN Qin GONG Xiaojiao YANG Chengxue(Implant Department, Stomatological Hospital Affiliated to Zunyi Medical College, Zunyi Guizhou 563003, Chin)

机构地区：[1]遵义医学院附属口腔医院种植科,贵州遵义563003

出　　处：《中国继续医学教育》2017年第21期85-88,共4页China Continuing Medical Education

基　　金：遵义医学院2012年度硕士启动科研基金(ZYKQS-2)

摘　　要：目的检测钴铬合金、银钯合金及纯钛金属全冠修复后犬牙龈细胞中Bcl-2和Bax基因m RNA的表达,探讨3种材料全冠修复对细胞凋亡的影响。方法选取5只成年雄性实验犬,将每只犬的牙弓分为4个区,以每区的尖牙为实验对象,分别行钴铬合金(A组)、银钯合金(B组)及纯钛(C组)金属全冠修复,第4区为空白对照(D组);分别于修复后1、2、3个月采集尖牙牙龈组织,用逆转录聚合酶链式反应(RT-PCR)法检测牙龈细胞中Bcl-2、Bax基因m RNA的表达并计算Bcl-2/Bax的比率。结果修复后3个月,A组Bcl-2/Bax的比率高于D组,差异有统计学意义(P<0.05);修复后2、3个月,B组Bcl-2/Bax的比率低于D组,差异有统计学意义(P<0.05);修复后1、2、3个月,C组Bcl-2/Bax的比率与D组相比,差异均无统计学意义(P>0.05)。结论钴铬合金抑制牙龈细胞凋亡,银钯合金促进牙龈细胞凋亡,而纯钛对牙龈细胞的凋亡无明显影响。Objective To observe the expression of Bcl-2 and Bax gene in gingival cells of the dogs＇ after restored with cobalt-chrome alloy full crown and palladium-silver alloy full crown and pure titanium full crown. Investigate the effects of three materials on apoptosis of the gingival cells. Methods 5 adult male dogs were chosen for the experiment, each dog＇s dental arch was divided into 4 sections. Each of the fang of each area was restored with cobalt-chrome alloy crown, palladium-silver alloy crown and pure titanium crown respectively, the fourth area is for control group. To collect the gingival tissue of fangs in 1, 2 and 3 months after restoration. The level of Bcl-2 and Bax gene expression were detected by RT-PCR. Results After 3 months restoration, the group A was superior to group D on the Bcl-2/Bax ratio with statistically significant difference（P〈0.05）. After 2 and 3 months restoration, the group B was inferior to group D on the Bcl- 2/Bax ratio with statistically significant difference （P〈0.05）. After l, 2 and 3 months restoration, the group C had no statistically significant difference with group D on the Bcl-2/Bax ration （P〉0.05）. Conclusion The cobalt- chromium alloy inhibits the apoptosis of gingival cells, the palladium-silver alloy promotes the apoptosis of gingival cells, while the pure titanium has no obvious effect on the apoptosis of gingival cells.

关 键 词：金属全冠 B淋巴细胞瘤/白血病-2 Bcl-2相关蛋白X 

分 类 号：R783[医药卫生—口腔医学]