槲皮素通过激活Kv1.5保护糖尿病大鼠冠脉损伤  

Quercetin protects coronary artery from injury induced by diabetes in rats by activating Kv1.5

作　　者：侯晓敏[1] 秦小江[2] 

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西医科大学公共卫生学院,山西太原030001

出　　处：《中国药理学通报》2017年第10期1442-1445,共4页Chinese Pharmacological Bulletin

基　　金：山西省高等学校科技创新项目(No 2017146;2017147);山西省青年科技研究基金(No 201701D221247;201701D221259);山西医科大学博士启动基金(No 03201510;03201521);山西医科大学青年基金(No 02201604;02201613)

摘　　要：目的研究槲皮素对糖尿病大鼠CA损伤的改善作用及该作用与Kv1.5的关系。方法 30只♂SD大鼠,随机分为3组:空白对照组、糖尿病组、"糖尿病+槲皮素"组。通过大鼠冠脉流量(coronary flow,CF)测定,观察槲皮素对糖尿病所致CF变化的影响;利用冠状动脉(coronary artery,CA)张力测定,观察槲皮素对糖尿病所致CA张力变化的影响;应用膜片钳记录CA血管平滑肌细胞(vascular smooth muscle cell,VSMC)电压依赖性钾通道(voltage gated potassium channel,Kv)电流及测定CA VSMC Kv1.5 mRNA表达水平,探讨槲皮素改善糖尿病所致CA损伤的机制。结果糖尿病组CF较空白组明显下降,糖尿病大鼠饮食中补充槲皮素,可使得其CF有所增加;膳食补充槲皮素可减弱糖尿病大鼠CA对KCl的收缩反应(P<0.05);与糖尿病组相比,"糖尿病+槲皮素"组CA对Kv阻断剂4-AP的收缩幅度明显降低;糖尿病组大鼠CA VSMC Kv电流较空白组明显降低(P<0.05),膳食补充槲皮素可减小其降低幅度;RT-PCR结果表明,Kv1.5 mRNA相对表达量空白组最高,"糖尿病+槲皮素"组次之,糖尿病组最少。结论槲皮素对糖尿病CA损伤有保护作用,该作用与激活Kv1.5存在一定相关性。Aim To research into the effect of quercetin on coronary artery（ CA） damage in diabetic rats and its relationship with Kv1. 5. Methods Thirty male rats were randomly divided into blank control group,diabetes group and diabetes group ＋ quercetin group. The effects of quercetin on heart coronary flow（ CF） in diabetes rat were observed by CF measurement; the effects of quercetin on CA tension in diabetes rat were detected by CA tension measurement. To investigate the mechanism of quercetin improving CA lesions caused by diabetes,Kv currents of CA VSMC in rats were recorded using whole cell patch clamp,and Kv1. 5 mRNA of CA VSMC was determined. Results Compared with normal group,CF of diabetic rats dropped significantly,and CF could increase with the supplement of quercetin in rat diet; the maximum contraction amplitude of CA in response to the contraction of KCl could be reduced with supplement of quercetin in diabetic rat dietary; compared with diabetes group,the contraction of CA from ＂diabetes ＋ quercetin＂to 4-AP significantly decreased; compared with blank control group,CA VSMC Kv currents of diabetes group had a significant decrease（ P 〈0. 05）,and dietary supplement of quercetin could improve the above changes; RT-PCR results indicated that the expression of Kv1. 5 mRNA on rat CA was the highest in control group,then in diabetes group and the lowest in ＂diabetes ＋ quercetin＂group. Conclusion Quercetin has protective effect on coronary muscle damage caused by diabetes,which maybe related to Kv1. 5 channel.

关 键 词：槲皮素 KV1.5 糖尿病 离子通道 冠状动脉 血管损伤 

分 类 号：R-332[医药卫生] R284.1