Nrf2-ARE信号通路在左乙拉西坦抗癫痫中的作用  

Effects of Nrf2-ARE signal path on levrtiracetam anti-epileptic and levrtiracetam on learning and memorizing ability

作　　者：吴可 赵文丽 李月影 邱长雨 施海静 张咏梅 

机构地区：[1]徐州医科大学麻醉学院,江苏徐州221004

出　　处：《中国药理学通报》2017年第10期1462-1466,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81171041);江苏省高校自然科学基金重点项目(No 13KJA320001);江苏省自然科学基金资助项目(No BK20161171);江苏省大学生创新创业训练计划(No 201610313039Y);江苏高校品牌专业建设工程资助项目(No PPZY2015A066)

摘　　要：目的探讨Nrf2-ARE信号通路在左乙拉西坦抗癫痫中的作用,以及应用左乙拉西坦对认知功能的影响。方法成年♂SD大鼠36只,250~300 g,随机分为生理盐水对照(control)组、戊四唑(1,5-pentamethylene-1H-tetrazole,PTZ)癫痫模型组、左乙拉西坦(levetiracetam,LEV)对照组以及左乙拉西坦治疗组,每组9只。以Morris水迷宫测试大鼠的空间学习记忆能力;用免疫印迹法测定海马组织中Nrf2、HO-1和NQO1蛋白表达量。结果与癫痫模型组相比,给予左乙拉西坦治疗的癫痫大鼠在Morris水迷宫测试中潜伏期明显缩短(P<0.05),海马Nrf2、HO-1、NQO1蛋白表达均明显增高(P<0.01)。结论左乙拉西坦能够改善癫痫大鼠的认知功能,其机制可能通过Nrf2-ARE通路,使HO-1、NQO1蛋白表达增多,起到抗癫痫的作用。Aim To explore the effects of Nrf2-ARE signal path on levrtiracetam anti-epileptic and levrtiracetam on learning and memorizing ability. Methods Thirty-six SD rats were divided into normal saline group,levrtiracetam group,model group and treatment group. Each group recruited nine rats. Tests of Morries water maze were given to the rats to evaluate their learning and memorizing ability. The protein expression of nuclear factor（ erythroid-derived2）-like2（ Nrf2）,heme oxygenase 1（ HO-1） and NAD（ P） H quinone oxidoreductase（ NQO1） were examined by Western blot. Results Compared with model group,levrtiracetam could shorten the plateau period in epileptic rats（ P 〈 0. 05）,and increase the expression of Nrf2 protein,HO-1 protein and NQO1 protein in hippocampus（ P〈 0. 05）. Conclusions Levrtiracetam could improve the learning and memorizing ability in epileptic rats. Levrtiracetam may increase the expression of HO-1 protein and NQO1 protein through the Nrf2-ARE pathway and play a part in antiepileptic effects.

关 键 词：癫痫 NRF2 ARE 信号通路 左乙拉西坦 认知功能 

分 类 号：R-332[医药卫生] R322.81